Optical phantoms of varying geometry based on thin building blocks with controlled optical properties
Optical phantoms of varying geometry based on thin building blocks with controlled optical properties
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DOI：
10.1117/1.3369003
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发表时间：
2010-03-01
影响因子：
3.5
通讯作者：
Faber, Dirk J.
中科院分区：
文献类型：
--
作者：
de Bruin, Daniel M.;Bremmer, Rolf H.;Faber, Dirk J.
当前在光学成像、测量技术和数据分析算法方面的创新表达了对可靠测试和比较方法的需求。我们提出了基于有机硅弹性体的光学幻影的设计和表征。通过加入绿色染料包括吸收，通过加入TiO(2)或SiO(2)颗粒包括散射。光学相干层析成像测量表明，在没有吸收剂的情况下，衰减系数与散射体浓度呈线性关系。非散射吸收模的透射光谱显示出与浓度线性相关的吸收系数。这两种类型的样品在6个月内都是稳定的。样品的共聚焦显微镜显示散射体均匀分布，尽管有一些聚类。基于厚度仅为50 μ m的层，我们制作了类似于流道的多层结构，（波浪状）皮肤结构，以及类似于人类视网膜的分层和弯曲的幻影。最后，我们展示了将金纳米颗粒纳入幻影的能力。总之，我们的幻影很容易制造，基于价格合理的材料，具有明确的和可控的厚度，折射率，吸收和散射系数，是均匀的，并且允许加入新型的纳米颗粒造影剂。我们相信我们的幻影满足了“理想”组织幻影的许多要求，并将特别适合于新型光学相干断层扫描应用。(C) 2010年光学仪器工程师学会。(DOI: 10.1117/1.3369003)
Current innovations in optical imaging, measurement techniques, and data analysis algorithms express the need for reliable testing and comparison methods. We present the design and characterization of silicone elastomer-based optical phantoms. Absorption is included by adding a green dye and scattering by adding TiO(2) or SiO(2) particles. Optical coherence tomography measurements demonstrate a linear dependence of the attenuation coefficient with scatterer concentration in the absence of absorbers. Optical transmission spectroscopy of the nonscattering absorbing phantoms shows a linear concentration dependent absorption coefficient. Both types of samples are stable over a period of 6 months. Confocal microscopy of the samples demonstrates a homogeneous distribution of the scatterers, albeit with some clustering. Based on layers with thicknesses as small as 50 mu m, we make multifaceted structures resembling flow channels, (wavy) skin-like structures, and a layered and curved phantom resembling the human retina. Finally, we demonstrate the ability to incorporate gold nanoparticles within the phantoms. In conclusion, our phantoms are easy to make, are based on affordable materials, exhibit well-defined and controllable thickness, refractive index, absorption, and scattering coefficients, are homogeneous, and allow the incorporation of novel types of nanoparticle contrast agents. We believe our phantoms fulfill many of the requirements for an "ideal" tissue phantom, and will be particularly suited for novel optical coherence tomography applications. (C) 2010 Society or Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3369003]