Limited MHC class I intron 2 repertoire variation in bonobos
Limited MHC class I intron 2 repertoire variation in bonobos
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DOI：
10.1007/s00251-017-1010-x
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发表时间：
2017-10-01
期刊：
影响因子：
3.2
通讯作者：
Bontrop, Ronald E.
中科院分区：
文献类型：
--
作者：
de Groot, Natasja G.;Heijmans, Corrine M. C.;Bontrop, Ronald E.
普通黑猩猩（Pan troglodytes）经历了一次选择性扫描，可能是由SIV样病毒引起的，该病毒针对它们的MHC I类库。基于MHC I类内含子2数据分析，这种选择性扫描发生在大约200 - 300万年前。因此，常见的黑猩猩有一个倾斜的MHC I类库，是丰富的同种异型，能够识别SIV蛋白质组的保守区域。倭黑猩猩（Pan paniscus）在大约150万到200万年前与普通黑猩猩有着共同的祖先。为了研究这种选择性扫描的特征是否也在倭黑猩猩中可检测到，通过桑格测序研究了总共包含29只动物的两组倭黑猩猩的MHC I类基因库。我们鉴定了14个Papa-A、20个Papa-B和11个Papa-C等位基因，其中分别有8个、5个和8个等位基因以前未报道过。在这个池的MHC I类变异，我们只回收2 Papa-A，3 Papa-B和6 Papa-C内含子2序列。与人类相比，倭黑猩猩似乎具有比普通黑猩猩更少的MHC I类内含子2谱系库。这支持了这样一种观点，即选择性扫荡可能早于普通黑猩猩和倭黑猩猩的物种形成。与普通黑猩猩相比，在倭黑猩猩中观察到的MHC I类内含子2谱系库的进一步减少可以通过创始效应或其他后续选择过程来解释。
Common chimpanzees (Pan troglodytes) experienced a selective sweep, probably caused by a SIV-like virus, which targeted their MHC class I repertoire. Based on MHC class I intron 2 data analyses, this selective sweep took place about 2-3 million years ago. As a consequence, common chimpanzees have a skewed MHC class I repertoire that is enriched for allotypes that are able to recognise conserved regions of the SIV proteome. The bonobo (Pan paniscus) shared an ancestor with common chimpanzees approximately 1.5 to 2 million years ago. To investigate whether the signature of this selective sweep is also detectable in bonobos, the MHC class I gene repertoire of two bonobo panels comprising in total 29 animals was investigated by Sanger sequencing. We identified 14 Papa-A, 20 Papa-B and 11 Papa-C alleles, of which eight, five and eight alleles, respectively, have not been reported previously. Within this pool of MHC class I variation, we recovered only 2 Papa-A, 3 Papa-B and 6 Papa-C intron 2 sequences. As compared to humans, bonobos appear to have an even more diminished MHC class I intron 2 lineage repertoire than common chimpanzees. This supports the notion that the selective sweep may have predated the speciation of common chimpanzees and bonobos. The further reduction of the MHC class I intron 2 lineage repertoire observed in bonobos as compared to the common chimpanzee may be explained by a founding effect or other subsequent selective processes.