Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ
Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ
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水稻脆秆突变体的代谢组学分析揭示了每个器官中每个突变体特异性的代谢模式
DOI：
10.1007/s11306-022-01958-9
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发表时间：
2022
期刊：
影响因子：
3.6
通讯作者：
Maki Kawai-Yamada
中科院分区：
文献类型：
--
作者：
Atsuko Miyagi;Kazuhisa Mori;Toshiki Ishikawa;Satoshi Ohkubo;Shunsuke Adachi;Masatoshi Yamaguchi;Taiichiro Ookawa;Toshihisa Kotake;Maki Kawai-Yamada
前言植物细胞壁在提供生理力量和抵御非生物胁迫方面起着重要作用。水稻脆秆突变体是一种细胞壁异常导致强度降低的突变体，已有研究表明其致病基因影响细胞壁组成。目的通过对水稻突变体叶片、节间、叶片中的初级代谢产物进行比较分析，研究突变体体内初级代谢产物的变化规律。使用CE-和LC-MS/MS测量水稻突变体和野生型对照的节。MS.使用代谢组学数据进行多变量分析。ResultsWe发现，在eachbc突变体的突变对代谢有不同的影响。例如，在bc 3和bc 1 bc 3突变体中观察到更高的草酸含量，这表明未用于细胞壁组分的剩余碳可能用于草酸合成。此外，共同的代谢变化，如糖核苷酸的节中的减少被发现inbc 1和Bc 6，其中致病基因参与纤维素accumulation.ConclusionThese结果表明，代谢分析的thebc突变体可以阐明致病基因的功能，并改善细胞壁组分的牲畜饲料或生物乙醇生产。
IntroductionPlant cell walls play an important role in providing physical strength and defence against abiotic stress. Ricebrittle culm(bc) mutants are a strength-decreased mutant because of abnormal cell walls, and it has been reported that the causative genes ofbcmutants affect cell wall composition. However, the metabolic alterations in each organ ofbcmutants have remained unknown.ObjectivesTo evaluate the metabolic changes in ricebcmutants, comparative analysis of the primary metabolites was conducted.MethodsThe primary metabolites in leaves, internodes, and nodes of ricebcmutants and wild-type control were measured using CE- and LC-MS/MS. Multivariate analyses using metabolomic data was performed.ResultsWe found that mutations in eachbcmutant had different effects on metabolism. For example, higher oxalate content was observed inbc3andbc1 bc3mutants, suggesting that surplus carbon that was not used for cell wall components might be used for oxalate synthesis. In addition, common metabolic alterations such as a decrease of sugar nucleotides in nodes were found inbc1andBc6,in which the causative genes are involved in cellulose accumulation.ConclusionThese results suggest that metabolic analysis of thebcmutants could elucidate the functions of causative gene and improve the cell wall components for livestock feed or bioethanol production.