An epidermal cell-derived cytokine triggers the in vivo synthesis of serum amyloid A by hepatocytes.
An epidermal cell-derived cytokine triggers the in vivo synthesis of serum amyloid A by hepatocytes.
复制标题
表皮细胞衍生的细胞因子触发肝细胞体内合成血清淀粉样蛋白 A。
作者：
M. Sztein;T. Luger;J. Oppenheim
脂多糖（LPS）刺激巨噬细胞产生许多密切相关的可溶性介质，包括白细胞介素1 （IL 1）、内源性热原（EP）和血清淀粉样蛋白a （SAA）诱导剂，它们能够诱导肝细胞产生SAA（一种急性期反应物）。角质形成细胞也产生可溶性介质，表皮细胞源性胸腺细胞活化因子（ETAF），这与IL - 1非常相似。本文报道的实验表明，未纯化的含ETAF制剂，以及经凝胶过滤（Sephacryl S-200）层析的ETAF， DEAE阴离子交换，苯基- sepharose或等电聚焦，都能诱导肝细胞在体内产生SAA。此外，ETAF和SAA诱导剂表现出相同的温度敏感性模式。这些观察结果进一步支持了ETAF、IL - 1、EP和SAA诱导剂由一组密切相关的介质组成的假设，并且非淋巴样细胞可以产生调节全身炎症反应的介质。
Lipopolysaccharide (LPS) stimulates macrophages to produce a number of closely related soluble mediators, including interleukin 1 (IL 1), endogenous pyrogen (EP), and serum amyloid A (SAA) inducer, which are able to induce hepatocyte production of SAA, an acute-phase reactant. Keratinocytes also produce a soluble mediator, epidermal cell-derived thymocyte-activating factor (ETAF), which is very similar to IL 1. The experiments reported here show that unpurified ETAF-containing preparations, as well as ETAF after chromatography by gel filtration (Sephacryl S-200), anion-exchange on DEAE, phenyl-Sepharose, or isoelectrofocusing, were all able to induce the in vivo production of SAA by hepatocytes. Additionally, ETAF and SAA inducer exhibited the same pattern of temperature sensitivity. These observations add further support to the hypothesis that ETAF, IL 1, EP, and SAA inducer comprise a group of closely related mediators and that nonlymphoid cells can produce mediators that can regulate systemic inflammatory reactions.