Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection
Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection
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DOI：
10.1177/002215540305100801
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发表时间：
2003-08-01
影响因子：
3.2
通讯作者：
Roth, KA
中科院分区：
文献类型：
--
作者：
Ness, JM;Akhtar, RS;Roth, KA
组织切片中生物相关蛋白质和抗原的常规免疫荧光检测通常受到相对较弱的信号的限制，这些信号在光照下会迅速褪色。我们开发了一种免疫组织化学方案，将酪胺信号放大的敏感性与量子点的光稳定性结合起来，以克服这些限制。这种简单的方法提供了一个敏感和稳定的荧光免疫组织化学替代标准的色原检测。
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.