Beta1 integrin mediation of myogenic differentiation: implications for satellite cell differentiation.
Beta1 integrin mediation of myogenic differentiation: implications for satellite cell differentiation.
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DOI：
10.1093/ps/83.2.245
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发表时间：
2004-02
期刊：
影响因子：
4.4
通讯作者：
S. Velleman;D. McFarland
中科院分区：
文献类型：
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作者：
S. Velleman;D. McFarland
整合素受体介导的成肌细胞-细胞外基质相互作用在骨骼肌发育中起着关键作用。在本研究中，以禽类遗传性肌肉无力，低分正常(LSN)为模型系统，进一步研究了β1整合素在成肌分化中的作用。在正常的卫星细胞分化过程中，β1整合素定位于伪足活动区和细胞接触点。相反，在LSN卫星细胞培养中，β1整合素主要在与细胞接触点无关的簇中观察到。LSN卫星细胞发生凋亡的同时，正常卫星细胞正在融合形成多核肌管。在肌肉发育过程中，β1整合素通过从β1 A到β1 D形式的选择性剪接进行调节。在胚胎和后胸大肌发育过程中，LSN中Beta1A和Beta1D变异的调控发生了变化。培养的正常和LSN肌源性卫星细胞不表达β1D整合素，但LSN卫星细胞表达的β1 A整合素浓度高于正常卫星细胞。综上所述，这些数据进一步证明了在肌肉生长和分化过程中，β1整合素亚基在调节细胞-细胞外基质信号转导通路中的重要性。
Myoblast-extracellular matrix interactions mediated by integrin receptors have been shown to play a pivotal role in skeletal muscle development. In the current study, the avian genetic muscle weakness, low score normal (LSN), which exhibits modified myotube and sarcomere structure and a reduction in beta1 integrin expression during satellite cell differentiation, was used as a model system to further investigate the role of beta1 integrins in myogenic differentiation. During normal satellite cell differentiation, the beta1 integrin was localized at areas of pseudopodial activity and points of cell contact. In contrast, in LSN satellite cell cultures the beta1 integrin was predominantly observed in clusters not associated with points of cell to cell contact. The LSN satellite cells underwent apoptosis at the same time normal satellite cells were fusing to form multinucleated myotubes. During muscle development, the beta1 integrin is regulated by alternative splicing from a beta1A to beta1D form. During both embryonic and posthatch pectoralis major muscle development, regulation of the beta1A and beta1D variants was altered in the LSN. Normal and LSN myogenic satellite cell cultures did not express the beta1D integrin, but the LSN satellite cells expressed higher concentrations of the beta1A integrin than did normal satellite cells. Taken together, these data further demonstrate the importance of the beta1 integrin subunit in the regulation of cell-extracellular matrix signal transduction pathways during muscle growth and differentiation.