Granulocyte collection by polymorphonuclear cell-targeting apheresis with medium-molecular-weight hydroxyethyl starch
Granulocyte collection by polymorphonuclear cell-targeting apheresis with medium-molecular-weight hydroxyethyl starch
复制标题
使用中等分子量羟乙基淀粉进行多形核细胞靶向分离术收集粒细胞
DOI：
10.1007/s12185-021-03207-6
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发表时间：
2021
影响因子：
2.1
通讯作者：
Kunisaki Yuya
中科院分区：
文献类型：
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作者：
Henzan Tomoko;Yamauchi Takuji;Yamanaka Ikumi;Sakoda Teppei;Semba Yuichiro;Hayashi Masayasu;Kikushige Yoshikane;Mishima Hiroyuki;Ishimura Masataka;Koga Yuhki;Miyamoto Toshihiro;Ohga Shouichi;Akashi Koichi;Maeda Takahiro;Kunisaki Yuya
粒细胞输注（GTX）是长期中性粒细胞减少症患者严重感染的治疗选择。通过献血者的单采收集有效的粒细胞需要从红细胞（RBC）中明确分离粒细胞，而高分子量（MW）羟乙基淀粉（HES）的输注促进了红细胞的沉淀。最近的研究表明，采用中- mw HES采集血液可以防止高- mw HES对供体的不良影响，但采用中- mw HES采集血液的理由尚未得到评估。为了验证中等mw HES的使用，我们首先对全血样本进行了实验，以确定高、中、低mw HES从红细胞中分离粒细胞的效率，并发现中等mw HES与高mw HES一样有效。我们还回顾了我们机构的粒细胞分离的临床数据，以评估粒细胞产量。粒细胞收集的回顾性分析显示，中等分子量HES的单采可产生足够的GTX粒细胞，供体贫血降低了收集效率。这些结果可能有助于我们建立一种更安全的针对多形核粒细胞的采血方法，作为高分子量HES的替代方法。
Granulocyte transfusion (GTX) is a therapeutic option for patients with prolonged neutropenia suffering from severe infections. Efficient granulocyte collection by apheresis from donors requires clear separation of granulocytes from red blood cells (RBCs), and infusion of high-molecular-weight (MW) hydroxyethyl starch (HES) facilitates RBC sedimentation. Recent research has shown that apheresis with medium-MW HES may prevent adverse effects of high-MW HES on donors, but the rationale for collection with medium-MW HES has yet to be evaluated. To validate the use of medium-MW HES, we first performed experiments with whole blood samples to determine how efficiently high-, medium- and low-MW HES separated granulocytes from RBCs, and found that medium-MW HES was just as efficient as high-MW HES. We also reviewed clinical data of granulocyte apheresis at our institution to evaluate granulocyte yields. Retrospective analysis of granulocyte collection revealed that apheresis with medium-MW HES yielded sufficient granulocytes for GTX and that donor anemia reduced collection efficiency. These results collectively may help us to establish a safer method for apheresis targeting polymorphonuclear granulocytes as an alternative to high-MW HES.