Aspergillus fumigatus rasA and rasB regulate the timing and morphology of asexual development
Aspergillus fumigatus rasA and rasB regulate the timing and morphology of asexual development
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DOI：
10.1016/j.fgb.2003.10.004
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发表时间：
2004-02-01
影响因子：
3
通讯作者：
Rhodes, JC
中科院分区：
文献类型：
--
作者：
Fortwendel, JR;Panepinto, JC;Rhodes, JC
rasA的表达在细粒曲霉孢子萌发中起重要作用。条件致病菌烟曲霉（Aspergillus fumigatus）的感染和无性发育都需要分生孢子萌发。因此，我们试图确定烟曲霉孢子萌发和无性发育对Ras蛋白的需求。第二个同系物，rasB，已经被确定为Ras基因的一个新亚类。每个基因的显性活性（DA）和显性阴性（DN）突变作为转基因引入原生质体。DArasA表达导致分生孢子减少，分生孢子畸形，有丝分裂进程改变，而DNrasA表达导致分生孢子萌发率显著降低。相反，DNrasB的表达稍微延迟了萌发的开始，并导致了分生孢子的发育。DArasB的表达导致条件减少。RasA和RasB在烟曲霉的营养生长和无性发育中起着不同但重叠的作用。(C) 2003 Elsevier Inc.版权所有。
Expression of rasA plays an important role in conidial germination in Aspergillus nidulans. Conidial germination is required to initiate both infection and asexual development in the opportunistic pathogen Aspergillus fumigatus. Therefore, we sought to determine the requirements for Ras proteins in conidial germination and asexual development of A. fumigatus. A second homolog, rasB, has been identified that characterizes a new subclass of Ras genes. Dominant active (DA) and dominant negative (DN) mutations of each gene were introduced into protoplasts as transgenes. DArasA expression led to reduced conidiation, malformed conidiophores, and altered mitotic progression, whereas expression of DNrasA caused a significant reduction in the rate of conidial germination. In contrast, expression of DNrasB slightly delayed the initiation of germination and caused the development of conidiophores in submerged culture. DArasB expression led to reduced conidiation. RasA and RasB appear to play different, but overlapping, roles in the vegetative growth and asexual development of A. fumigatus. (C) 2003 Elsevier Inc. All rights reserved.