Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection
Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection
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DOI：
10.1016/j.virol.2005.06.010
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发表时间：
2005-09-01
期刊：
影响因子：
3.7
通讯作者：
Onuma, M
中科院分区：
文献类型：
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作者：
Konnai, S;Usui, T;Onuma, M
此前，我们发现实验性感染牛白血病病毒（BLV）的绵羊体内肿瘤坏死因子α（TNF）-α表达上调，且TNF受体失衡。为了研究 TNF-α 诱导的不同反应，在本研究中，我们检测了 TNF-α 诱导的增殖反应以及来自未感染 BLV 的牛和患有白血病 (AL) 或持续性淋巴细胞增多症 (PL) 的 BLV 感染牛的外周血单核细胞 (PBMC) 上两种不同 TNF 受体的表达水平。在重组牛 TNF-α (rTNF-α) 存在下，从 PL 牛中分离的 PBMC 的增殖反应显着高于来自 AL 牛和未感染牛的细胞，并且来自 PL 牛的细胞表达的 TNF 受体 II 型 (TNF-RII) mRNA 水平显着高于来自 AL 和 BLV 未感染牛的细胞。 TNF-RI mRNA 水平没有发现差异。 PL牛中大多数表达TNF-RII的细胞是CD5(+)或sIgM(+)细胞，这些细胞对TNF-α诱导的细胞凋亡具有抵抗力。此外，原病毒载量和TNF-RII mRNA水平的变化以及TNF-α诱导的增殖和TNF-RII mRNA水平之间存在显着正相关。这些数据表明，TNF 受体表达的不平衡可能至少部分促进 BLV 感染中淋巴细胞增多的进展。 (c) 2005 Elsevier Inc. 保留所有权利。
Previously, we found an up-regulation of tumor necrosis factor alpha (TNF)-alpha and an imbalance of TNF receptors in sheep experimentally infected with bovine leukemia virus (BLV). In order to investigate the different TNF-alpha-induced responses, in this study we examined the TNF-alpha-induced proliferative response and the expression levels of two distinct TNF receptors on peripheral blood mononuclear cells (PBMC) derived from BLV-uninfected cattle and BLV-infected cattle that were aleukemic (AL) or had persistent lymphocytosis (PL). The proliferative response of PBMC isolated from those cattle with PL in the presence of recombinant bovine TNF-alpha (rTNF-alpha) was significantly higher than those from AL cattle and uninfected cattle and the cells from PL cattle expressed significantly higher mRNA levels of TNF receptor type II (TNF-RII) than those from AL and BLV-uninfected cattle. No difference was found in TNF-RI mRNA levels. Most cells expressing TNF-RII in PL cattle were CD5(+) or sIgM(+) cells and these cells showed resistance to TNF-a-induced apoptosis. Additionally, there were significant positive correlations between the changes in provirus load and TNF-RII mRNA levels, and TNF-alpha-induced proliferation and TNF-RII mRNA levels. These data suggest that imbalance in the expression of TNF receptors could at least in part contribute to the progression of lymphocytosis in BLV infection. (c) 2005 Elsevier Inc. All rights reserved.