THE DOMINANT ROLE OF THE LIVER IN PLASMA PROTEIN SYNTHESIS - A DIRECT STUDY OF THE ISOLATED PERFUSED RAT LIVER WITH THE AID OF LYSINE-EPSILON C-14
THE DOMINANT ROLE OF THE LIVER IN PLASMA PROTEIN SYNTHESIS - A DIRECT STUDY OF THE ISOLATED PERFUSED RAT LIVER WITH THE AID OF LYSINE-EPSILON C-14
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DOI：
10.1084/jem.94.5.431
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发表时间：
1951-01-01
影响因子：
15.3
通讯作者：
BALE, WF
中科院分区：
文献类型：
--
作者：
MILLER, LL;BLY, CG;BALE, WF
用含氨基酸和赖氨酸-[epsilon]-C14的含氧血灌流大鼠肝脏的直接研究表明，肝脏几乎合成了所有的血浆纤维蛋白原，白蛋白部分，以及可能超过80%的血浆球蛋白部分。分离的灌流肝脏在蛋白质合成方面的反应在定性和定量上与完整动物相似，特别是在(A)区分天然L赖氨酸和D-赖氨酸的能力，(B)转化为二氧化碳的同位素氨基酸的百分比，(C)用于肝脏和血浆蛋白质合成的百分比。与报道的组织匀浆、肉末和切片的结果进行了比较和对比。
A direct study of the isolated rat liver perfused with oxygenated blood containing amino acids and lysine-[epsilon] -C14 indicated that the liver synthesizes practically all the plasma fibrinogen, the albumin fraction, and probably more than 80% of the plasma globulin fraction. The response of the isolated perfused liver in protein synthesis is qualitatively and quantitatively analogous to that of the intact animal, notably in (a) the ability to discriminate between natural L-lysine and D-lysine, (b) the per cent of isotopic amino acid converted to CO2, (c) the per cent utilized in liver and plasma protein synthesis. The results obtained with the perfused liver are compared and contrasted with those reported for tissue homogenates, minces, and slices.