Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles
Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles
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DOI：
10.1007/s10529-013-1368-2
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发表时间：
2014-02-01
影响因子：
2.7
通讯作者：
Deguchi, Shinji
中科院分区：
文献类型：
--
作者：
Saito, Akira C.;Matsui, Tsubasa S.;Deguchi, Shinji
细胞片可用于组织再生医学。现在，片材内的细胞对齐是下一代这项技术的关键因素。没有随机单元取向的各向异性单元片通常使用特殊的设施和设备用光刻、微制造的基底来生产。在这里，我们展示了一个更容易的方法来制造各向异性基板。我们局部变形的弹性膜的一部分，同时氧化的表面，以创建微皱纹，以及使粘附到细胞外基质。局部加载的方法使得可以根据由可控变形确定的应变在单个膜片内将细胞定向在受控方向上。该技术潜在地实现了用于目标兼容细胞对准的微皱纹的通用设计。
Sheets of cells can be used for tissue regenerative medicine. Cell alignment within the sheet is now a key factor in the next generation of this technology. Anisotropic cell sheets without random cell orientations have been conventionally produced with photolithographically, microfabricated substrates using special facilities and equipment. Here we demonstrate a more accessible approach to the fabrication of anisotropic substrates. We locally deformed part of an elastic membrane and simultaneously oxidized the surface to create microwrinkles as well as to enable adhesion to the extracellular matrix. The approach with the local loading made it possible to orient cells in controlled directions within a single membrane sheet depending on the strains determined by the controllable deformation. This technique potentially enables a versatile design of microwrinkles for target-compatible cell alignments.