Killing Dental Pathogens Using Antibacterial Graphene Oxide
Killing Dental Pathogens Using Antibacterial Graphene Oxide
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DOI：
10.1021/acsami.5b01069
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发表时间：
2015-03-11
影响因子：
9.5
通讯作者：
Tang, Zisheng
中科院分区：
文献类型：
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作者：
He, Jianliang;Zhu, Xiaodan;Tang, Zisheng
龋病和牙周病与变形链球菌、牙龈卟啉单胞菌和具核梭杆菌等微生物有着密切的关系。氧化石墨烯（GO）作为石墨烯的衍生物，在生物、医学等诸多领域发挥着重要作用。特别是，它被认为是一种有前途的抗菌纳米材料。在这项研究中，我们专注于GO对牙科病原菌的抗菌性能。通过MTT还原试验、菌落形成单位（CFU）计数、生长曲线观察、活/死荧光染色和激光共聚焦显微镜观察，发现GO纳米片对口腔致病菌的生长具有良好的抑制作用。透射电子显微镜（TEM）图像显示，细菌的细胞壁和膜失去了完整性，细胞内的内容物泄漏后，他们处理GO。因此，GO纳米片将是一种有效的抗菌材料，对牙科病原菌和潜在的应用在牙科保健和治疗是有前途的。
Dental caries and periodontal diseases have a close relationship with microbes such as Streptococcus mutans, Porphyromonas gingivalis and Fusobacterium nucleatum. Graphene oxide (GO), as the derivative of graphene, plays an important role in many areas including biology and medicine. In particular, it has been known as a promising antimicrobial nanomaterial. In this study, we focused on the antimicrobial property of GO against dental pathogens. With the utilization of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduced test, colony forming units (CFU) counting, growth curve observation, live/dead fluorescent staining, and confocal laser scanning microscopy (CLSM), we found GO nanosheets were highly effective in inhibiting the growth of dental pathogens. Transmission electron microscopy (TEM) images revealed that the cell wall and membrane of bacteria lost their integrity and the intracellular contents leaked out after they were treated by GO. Therefore, GO nanosheets would be an effective antibacterial material against dental pathogens and the potential applications in dental care and therapy are promising.