Formation of Primordial Follicles and Immunolocalization of PTEN, PKB and FOXO3A Proteins in the Ovaries of Fetal and Neonatal Pigs
Formation of Primordial Follicles and Immunolocalization of PTEN, PKB and FOXO3A Proteins in the Ovaries of Fetal and Neonatal Pigs
复制标题
胎猪和新生猪卵巢中原始卵泡的形成和 PTEN、PKB 和 FOXO3A 蛋白的免疫定位
DOI：
10.1262/jrd.09-094h
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发表时间：
2010-02-01
影响因子：
1.8
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Ding, Wei;Wang, Wei;Taya, Kazuyoshi
原始卵泡的组装以及随后的原始卵泡向初级卵泡的发育和转变是卵巢生物学中的关键过程。为了研究猪胎儿和新生儿卵泡的形成和发育，本研究从中国著名的地方猪品种二花脸猪的卵巢中获得了从出生后50天到出生后1天的卵巢样品。胚胎和新生猪卵巢的形态学变化表明，卵巢是最早可识别的配子细胞。结果表明，在70 ~ 90d，卵巢比例由98.4%下降到25.69%，单卵泡比例由1.6%上升到74.4%。原始卵泡的比例在产后70 ~ 90天增加，但从产后90天到产后1天下降。结果表明，原始卵泡形成的关键时期是产后70 ~ 90 d，原始卵泡向初级卵泡转化的主要时期是产后90 ~ 1 d。本实验还通过免疫组织化学和免疫印迹技术检测了PTEN、PKB和FOXO 3A蛋白在猪卵巢中的定位。结果表明，PTEN、PKB和FOXO 3A在原始至次级卵泡的卵巢生殖细胞、卵母细胞胞质和卵泡颗粒细胞中均有表达，且在颗粒细胞中表达较弱，在卵母细胞中表达较强。PTEN、FOXO 3A和PKB的不同染色模式表明，这些蛋白质在胎儿和新生猪卵泡形成和发育过程中以阶段和细胞特异性的方式表达。
The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in Our Current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.69% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and I day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage- and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig.