Site-specific reflex response of ubiquitin to loop insertions.
Site-specific reflex response of ubiquitin to loop insertions.
复制标题
泛素对环插入的位点特异性反射反应。
DOI：
10.1016/j.jmb.2005.07.012
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Robertson,AndrewD
中科院分区：
文献类型：
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作者：
Ferraro,DebraM;Hope,ErinK;Robertson,AndrewD
通过同源建模预测蛋白质中插入的结构效应仍然是一个挑战。为了研究插入的构象适应的分子基础，通过在两个不同的位点引入5个不同的插入片段（大小从5到11个残基不等），产生了10个泛素突变体。大多数插入序列来自结构上同源的泛素样蛋白的同源位置;为了测试序列特异性，插入到泛素的同源和非同源位点。从NMR数据的结构推断表明，每个插入位点显示出对插入的反射反应：插入的序列对结构适应的影响比插入的位点小得多。此外，每个位点以独特但一致的方式响应插入。对于给定的插入位点，不同的插入序列产生不同的稳定性，但稳定性与序列之间的关系尚不清楚。然而，对于给定位点中的所有插入，稳定性的变化相似。
Predicting the structural effects of insertions in proteins by homology modeling remains a challenge. To investigate the molecular basis for conformational adaptations to insertions, ten mutants of ubiquitin were generated by introducing five different inserts, varying from five to 11 residues in size, at two different sites. Most insertion sequences were derived from homologous positions in structurally homologous ubiquitin-like proteins; to test sequence specificity, insertions were made into both homologous and non-homologous sites in ubiquitin. Structural inferences from NMR data suggest that each insertion site shows a reflex response to insertions: the sequence of the insertion has much less impact on structural adaptations than does the site of the insertion. Further, each site responds to insertions in a unique but consistent manner. For a given insertion site, different inserted sequences give rise to different stabilities, but the relationship between stability and sequence is not yet clear. However, the change in stability is similar for all insertions in a given site.