Effects of anions on ATP-induced [Ca2+], increase in NG108-15 cells.
Effects of anions on ATP-induced [Ca2+], increase in NG108-15 cells.
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DOI：
10.1254/jjp.89.302
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发表时间：
2002-07
期刊：
影响因子：
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通讯作者：
T. Watano;I. Matsuoka;K. Ogawa;J. Kimura
中科院分区：
文献类型：
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作者：
T. Watano;I. Matsuoka;K. Ogawa;J. Kimura
我们通过检测三磷酸腺苷对负载Fura-2的NG108-15和PC12细胞内钙离子浓度([Ca~(2+)]i)的影响来研究阴离子对不同P2受体的影响。在NG108-15细胞中，100微米和1 mM的ATP分别引起一过性和持续性的[Ca~(2+)]i升高。U-73122对前者有抑制作用，而对后者无抑制作用，提示前者通过P2Y2受体抑制，后者通过P2X7受体抑制。当外源性Cl-被其他阴离子取代时，由P2Y2受体介导的[Ca~(2+)]_i升高不变，但由P_2X7受体介导的[Ca~(2+)]_i升高按天冬氨酸->；甲烷磺酸>；Cl->；BR>；或=I-的顺序变化。在PC12细胞中，由P2Y2和P2X2受体介导的瞬时[Ca~(2+)]i升高不受各种阴离子的影响。这些结果表明，阴离子的调节是P2X7受体所特有的，而在P2Y2和P2X2受体中没有发生。这可能是因为ATP与P2X7受体结合的机制可能不同于与其他P2受体的结合机制。
We investigated the effects of anions on different P2 receptors by measuring ATP-induced increase in intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded NG108-15 and PC12 cells. In NG108-15 cells, ATP at 100 microM and 1 mM induced a transient and a sustained [Ca2+]i increase, respectively. The former, but not the latter, was inhibited by U-73122, indicating that the former was via the P2Y2 receptor and the latter via the P2X7 receptor. When external Cl- was replaced by other anions, the [Ca2+]i increase mediated by the P2Y2 receptor was not changed, but that mediated by the P2X7 receptor varied in the order of aspartate- > methanesulfonate > Cl- > Br > or = I-. In PC12 cells, transient [Ca2+]i increases mediated by the P2Y2 and P2X2 receptors were not affected by various anions. These results suggest that modulation by anions is unique to the P2X7 receptor and does not occur in P2Y2 and P2X2 receptors. This may be because the mechanism of ATP binding to the P2X7 receptor may be different than that to other P2 receptors.