Mediators of immediate hypersensitivity in nasal secretions during natural colds and rhinovirus infection.
Mediators of immediate hypersensitivity in nasal secretions during natural colds and rhinovirus infection.
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自然感冒和鼻病毒感染期间鼻分泌物中立即超敏反应的介质。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
J. Gwaltney
中科院分区：
文献类型：
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作者：
P. Eggleston;J. Hendley;J. Gwaltney
为了检验病毒性呼吸道感染通过激活粘膜肥大细胞释放对脉管系统和粘膜腺体有活性的介质而引起症状的假设，在自然感冒和鼻病毒感染期间评估了鼻分泌物中组胺的存在。用生理盐水冲洗鼻腔或用力吹入烧杯收集分泌物，并用荧光分光光度法测定组胺。在未感染受试者的吹气分泌物中，个体之间观察到较大的变化（范围为3 +/-2至59 +/-32 ng/ml），并且在给定受试者中每天观察到同样大的变化。鼻病毒和甲型流感感染并没有显著改变这些浓度。一般来说，无论是鼻腔分泌物和鼻腔冲洗，组胺浓度往往是较低的感染过程中。浓度的另一个预先形成的肥大细胞介质，TAME酯酶，也没有在感染过程中升高。因此，这些数据不支持肥大细胞活化发生在鼻病毒感染的假设。
To test the hypothesis that viral respiratory infections cause symptoms by activating mucosal mast cells to release mediators active on vasculature and mucosal glands, the presence of histamine in nasal secretions was assessed during natural colds and rhinovirus infections. Secretions were collected either with saline washes of the nasal cavity or by forcibly blowing into a beaker, and histamine was assayed spectrofluorometrically. In blown secretions from uninfected subjects, large variations were seen between individuals (ranging from 3 +/- 2 to 59 +/- 32 ng/ml), and equally large variations were seen from day to day in given subjects. Infection with rhinovirus and with influenza A did not change these concentrations significantly. In general, both with blown nasal secretions and with nasal washes, histamine concentrations tended to be lower during infection. Concentration of another preformed mast cell mediator, TAME-esterase, also was not elevated during infection. Thus, these data do not support an hypothesis that mast cell activation occurs during rhinovirus infections.