Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine
Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine
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DOI：
10.1111/j.1365-2796.2009.02185.x
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发表时间：
2010-01-01
影响因子：
11.1
通讯作者：
Okano, T.
中科院分区：
文献类型：
--
作者：
Elloumi-Hannachi, I.;Yamato, M.;Okano, T.
细胞片技术（CST）是基于使用温敏聚合物，聚（N-异丙基丙烯酰胺）（PIPAAm）。PIPAAms的表面以使其典型厚度< 100 nm的方式配制。在这篇综述中，我们首先关注PIPAAm接枝表面制备和功能化的方法对于能够收获功能细胞片层以进一步移植是如何重要的。然后，我们提出了组织模拟和三维重建的组织在体外方面。最后，我们给出了该技术的临床应用和临床相关的动物实验，如心肌病，视力，牙周病，食管溃疡和1型糖尿病的概述。
Cell sheet technology (CST) is based on the use of thermoresponsive polymers, poly(N-isopropylacrylamide) (PIPAAm). The surface of PIPAAms is formulated in such a way as to make its typical thickness < 100 nm. In this review, we first focus on how the methods of PIPAAm-grafted surface preparations and functionalization are important to be able to harvest a functional cell sheet, to be further transplanted. Then, we present aspects of tissue mimics and three-dimensional reconstruction of a tissue in vitro. Finally, we give an overview of clinical applications and clinically relevant animal experimentations of the technology, such as cardiomyopathy, visual acuity, periodonty, oesophageal ulcerations and type 1 diabetes.