DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.
DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.
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DOI：
10.1093/nar/gkx067
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发表时间：
2017-04-07
影响因子：
14.9
通讯作者：
Dryden DTF
中科院分区：
文献类型：
--
作者：
Cooper LP;Roberts GA;White JH;Luyten YA;Bower EKM;Morgan RD;Roberts RJ;Lindsay JA;Dryden DTF
金黄色葡萄球菌表现出克隆群体结构，在不同谱系之间的水平基因转移是极其罕见的。这在一定程度上是由于I型DNA限制性修饰（RM）系统的存在，该系统的通用名称为Sau1，它在不同谱系的基因组上的特定靶序列上保持不同的甲基化模式。我们已经确定了在广泛流行的金黄色葡萄球菌谱系中存在的Sau1 I型RM系统识别的靶序列，并将识别的序列分配到RM酶的特定靶识别域。我们使用一系列纯化酶的生化分析和基因组DNA的单分子实时测序来确定这些靶序列及其甲基化模式。了解Sau1的主要靶序列将有助于合成新的载体，用于转化这种“不可转化”细菌的最常见谱系。
Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this ‘untransformable’ bacterium.