DEATH IN VENTRICULAR-FIBRILLATION INDUCED BY ISOPROTERENOL IN DOCA-SALT PRETREATED RATS PRECEDED BY CHANGES IN MYOCARDIAL ELECTROLYTES
DEATH IN VENTRICULAR-FIBRILLATION INDUCED BY ISOPROTERENOL IN DOCA-SALT PRETREATED RATS PRECEDED BY CHANGES IN MYOCARDIAL ELECTROLYTES
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DOI：
10.1016/0024-3205(83)90818-4
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发表时间：
1983-01-01
期刊：
影响因子：
6.1
通讯作者：
GUIDERI, G
中科院分区：
文献类型：
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作者：
GUIDERI, G
在对照组和醋酸脱氧皮质酮（DOCA）-盐处理的大鼠中测定血清和组织中Na、K、Mg和Ca的含量，以确定电解质变化是否先于室颤中异丙肾上腺素诱导的死亡的发展。将对照雄性Sprague道利大鼠皮下注射。A组给予生理盐水，B组给予放线菌素D（0.1mg/kg），每日1次，连续4d。其余大鼠植入DOCA 20 mg，饮用生理盐水，注射生理盐水（C组）或放线菌素D（D组），每日1次，共4 d。在实验的第一部分中，当用异丙肾上腺素（150 μ g/kg，s.c.）6天后，D组15只大鼠中有13只在29.1 ± 1.5天内死亡。15.0 min（平均值±）SD）。心肌Na+升高（48.8 ± 0.01）。3.8与36.3 .+-. 1.9)K含量下降（60.4 ± 0.001）。3.4与70.6 .+-相比。3.3，meq/kg湿重，平均值±。SD）的大鼠死亡的异丙肾上腺素。在实验的第二部分中，从对照和DOCA-盐水预处理的大鼠中移除血清和组织，然后在异丙肾上腺素后20分钟死于室颤。DOCA-盐水预处理的大鼠高钠血症和低钾血症，骨骼肌中的Na和K比对照组大鼠高。异丙肾上腺素引起大鼠低钾血症，但仅使D组大鼠心肌Na含量升高，K含量降低，更易死于室颤。心肌电解质变化先于室颤发作，可能与心律失常的发生有关。
Serum and tissue content of Na, K, Mg and Ca was determined in controls and desoxycorticosterone acetate (DOCA)-salt treated rats to determine whether electrolyte changes preceded the development of isoproterenol-induced death in ventricular fibrillation. Control male Sprague Dawley rats were injected s.c. with saline (group A) or actinomycin D (0.1 mg/kg; group B) once daily for 4 days. Other rats received 20 mg DOCA by implantation, drank normal saline and were injected with saline (group C) or actinomycin D (group D) once daily for 4 days. In the 1st part of the experiment, none of 15 rats from group C died when challenged with isoproterenol (150 .mu.g/kg, s.c.) 6 days later; 13 of 15 rats from group D died within 29.1 .+-. 15.0 min (mean .+-. SD) from isoproterenol injection. Myocardial Na was elevated (48.8 .+-. 3.8 vs. 36.3 .+-. 1.9) and K decreased (60.4 .+-. 3.4 vs. 70.6 .+-. 3.3, meq/kg wet wt, mean .+-. SD) in rats that had succumbed to isoproterenol. In the 2nd part of the experiment serum and tissues were removed from control and DOCA-saline pretreated rats before they died in ventricular fibrillation, 20 min after isoproterenol. DOCA-saline pretreated rats were hypernatremic and hypokalemic and exhibited higher Na and lower K in skeletal muscle than control rats. Isoproterenol elicited hypokalemia in all rats, but it only elevated Na and decreased K content in the myocardium of rats of group D, that were more prone to die in ventricular fibrillation. Myocardial electrolyte changes preceded the onset of ventricular fibrillation and may have been associated with the development of this dysrhythmia.