Generation of intestinal T cells from progenitors residing in gut cryptopatches
Generation of intestinal T cells from progenitors residing in gut cryptopatches
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DOI：
10.1126/science.280.5361.275
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发表时间：
1998-04-10
期刊：
影响因子：
56.9
通讯作者：
Ishikawa, H
中科院分区：
文献类型：
--
作者：
Saito, H;Kanamori, Y;Ishikawa, H
从c-kit阳性（c-kit（+））但谱系标志物阴性（Lin（-））的小肠CP分离的细胞在体内转移或组织移植到严重联合免疫缺陷小鼠中后产生T细胞受体（TCR）α β和TCR γ δ肠上皮内T细胞。与此相反，来自派伊尔结和肠系膜淋巴结的细胞，属于同一肠道免疫区室，但缺乏c-kit（+）Lin（-）细胞，却不能这样做。这些发现和电镜分析结果提供了CP中局部肠道T细胞前体发育的证据。
Cryptopatches (CPs) are part of the murine intestinal immune compartment, Cells isolated from CPs of the small intestine that were c-kit positive (c-kit(+)) but lineage markers negative (Lin(-)) gave rise to T cell receptor (TCR) alpha beta and TCR gamma delta intestinal intraepithelial T cells after in vivo transfer or tissue engraftment into severe combined immunodeficient mice. In contrast, cells from Peyer's patches and mesenteric lymph nodes, which belong in the same intestinal immune compartment but lack c-kit(+)Lin(-) cells, failed lo do so, These findings and results of electron microscopic analysis provide evidence of a local intestinal T cell precursor that develops in the CPs.