Fluorescence in the tandem scanning microscope
Fluorescence in the tandem scanning microscope
复制标题
串联扫描显微镜中的荧光
DOI：
10.1111/j.1365-2818.1990.tb02945.x
复制
发表时间：
1990
影响因子：
2
通讯作者：
Timothy F. Watson
中科院分区：
文献类型：
--
作者：
A. Boyde;Sheila J. Jones;M. L. Taylor;LA Wolfe;Timothy F. Watson
我们的研究表明，荧光模式在串联扫描显微镜（TSM：直视共焦显微镜）和共焦扫描激光显微镜（CSLM）中都可以发挥良好的效果。所展示的应用表明 TSM 的两大优势是实时查看和真实色彩，从而可以更快地使用和解释。 CSLM 是互补的，而不是竞争的，目前对于低水平荧光工作来说更加复杂。这对于可用的 TSM 同样是可能的，但需要使用 CCD 相机和图像处理系统进行进一步开发。
Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real‐time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low‐level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image‐processing systems.