Genetic conservation of Highlands J viruses.
Genetic conservation of Highlands J viruses.
复制标题
Highlands J 病毒的遗传保护。
DOI：
10.1006/viro.1996.0203
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Weaver,SC
中科院分区：
文献类型：
--
作者：
Cilnis,MJ;Kang,W;Weaver,SC
我们通过对 1952 年至 1994 年间分离的 19 个病毒株产生的 PCR 产物进行测序，研究了蚊媒甲病毒 Highlands J (HJ) 病毒的分子进化。包括 E1 基因部分和 3' 非翻译区在内的 1200 个核苷酸的序列显示，进化速度相对较慢，估计每年每个核苷酸有 0.9–1.6 × 10−4 次取代。系统发育树表明所有 HJ 病毒都源自一个共同的祖先，并表明北美存在一个显性谱系。然而，两个或多个次要谱系可能同时传播数年至数十年。从患有脑炎的马中分离出的毒株与最近的火鸡疫情有关，与同一时期在其他地点分离出的毒株在系统发育上没有区别。我们的研究结果与我们之前获得的另一种北美甲病毒东部马脑脊髓炎病毒的结果非常相似，Highlands J 与该病毒具有相同的主要蚊子和鸟类宿主、地理分布和生态。这些结果支持了以下假设：传播季节的持续时间影响虫媒病毒的进化速率，脊椎动物宿主的流动性影响遗传多样性。
We studied molecular evolution of the mosquito-borne alphavirus Highlands J (HJ) virus by sequencing PCR products generated from 19 strains isolated between 1952 and 1994. Sequences of 1200 nucleotides including portions of the E1 gene and the 3′ untranslated region revealed a relatively slow evolutionary rate estimated at 0.9–1.6 × 10−4substitutions per nucleotide per year. Phylogenetic trees indicated that all HJ viruses descended from a common ancestor and suggested the presence of one dominant lineage in North America. However, two or more minor lineages probably circulated simultaneously for periods of years to a few decades. Strains isolated from a horse suffering encephalitis, and implicated in a recent turkey outbreak, were not phylogenetically distinct from strains isolated in other locations during the same time periods. Our findings are remarkably similar to those we obtained previously for another North American alphavirus, eastern equine encephalomyelitis virus, with which Highlands J shares primary mosquito and avian hosts, geographical distribution, and ecology. These results support the hypotheses that the duration of the transmission season affects arboviral evolutionary rates and vertebrate host mobility influences genetic diversity.