Evaluation of diffusional anisotropy and microscopic structure in skeletal muscles using magnetic resonance
Evaluation of diffusional anisotropy and microscopic structure in skeletal muscles using magnetic resonance
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DOI：
10.1016/j.mri.2005.09.009
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发表时间：
2006-01-01
影响因子：
2.5
通讯作者：
Ueno, S
中科院分区：
文献类型：
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作者：
Saotome, T;Sekino, M;Ueno, S
脉冲梯度自旋回波（PGSE）核磁共振（NMR）方法用于检测水分子在生物组织中的扩散。因为组织通常具有扩散各向异性，所以它们的扩散性质由张量表示。在这项研究中，我们评估的扩散各向异性和微观结构，在萎缩的骨骼肌使用PGSE核磁共振方法。切断12只9周龄大鼠的左侧坐骨神经。神经切断术导致左侧腓肠肌神经源性肌萎缩。在神经切断术后2、4和8周，从位于左侧腓肠肌的2 x 2 x 2 mm（3）体素中选择性采集磁共振信号。从信号计算扩散张量、平均扩散率（MD）和分数各向异性（FA）。本文从坦纳方程出发，导出了肌肉中扩散的理论模型。通过将模型拟合到测量的信号来估计肌纤维直径。正常大鼠作为对照组也进行了测量。对照组和失神经组之间的MD和估计的细胞内扩散系数无显着差异。失神经组FA值明显高于对照组（P
The pulsed-gradient spin-echo (PGSE) nuclear magnetic resonance (NMR) method is used for detecting the diffusion of water molecules in biological tissues. Because tissues generally have diffusional anisotropy, their diffusion properties are denoted by a tensor. In this study, we evaluated the diffusional anisotropy and microscopic structure in atrophied skeletal muscles using the PGSE NMR method. The left sciatic nerve was severed in twelve 9-week-old rats. Neurotomy caused neurogenic muscular atrophy at the left gastrocnermius. At 2, 4 and 8 weeks after neurotomy, magnetic resonance signals were selectively acquired from a 2 x 2 x 2 mm(3) voxel, which was located on the left gastrocnemius. The diffusion tenser, the mean diffusivity (MD) and the fractional anisotropy (FA) were calculated from the signals. A theoretical model of the diffusion in muscles was derived from Tanner's equation. The muscle fiber diameter was estimated by fitting the model to the measured signals. The measurements were also performed for normal rats as controls. No significant difference was found in the MD and the estimated intracellular diffusion coefficient between the control group and the denervated group. The denervated group had significantly higher FA compared with the control group (P