Taurine prevents Β-glycerophosphate-induced calcification in cultured rat vascular smooth muscle cells
Taurine prevents Β-glycerophosphate-induced calcification in cultured rat vascular smooth muscle cells
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DOI：
10.1007/s00380-003-0744-6
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发表时间：
2004-05
影响因子：
1.5
通讯作者：
Juxiang Li;Baohong Zhang;Zhiyu Huang;Shu-heng Wang;Chao-shu Tang;Junbao Du
中科院分区：
文献类型：
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作者：
Juxiang Li;Baohong Zhang;Zhiyu Huang;Shu-heng Wang;Chao-shu Tang;Junbao Du
血管钙化是动脉粥样硬化中常见的异位钙化。由于牛磺酸对心血管疾病具有保护作用，本研究在体外培养的钙化大鼠血管平滑肌细胞（VSMCs）上观察了牛磺酸对血管钙化的影响。在牛磺酸存在和不存在的情况下，检测VSMCs的成骨细胞分化、钙化和增殖。碱性磷酸酶（ALP）、细胞钙含量和45 Ca积累作为成骨细胞分化和钙化的指标。VSMC与Β-甘油磷酸孵育10天诱导成骨细胞样形态学变化。ALP活性增强。细胞内钙含量和~（45）Ca摄取增加。用Β-甘油磷酸盐处理证明这些VSMC的钙化。与这些改变相关，通过细胞计数、[3 H]胸苷（[3 H]TdR）和[3 H]亮氨酸（[3 H]Leu）掺入检测的细胞增殖在这些钙化的VSMC中也增加。20 mmol/l牛磺酸在早期和晚期处理后均降低钙含量、45 Ca 2+摄取和ALP活性，其中还注意到钙化的VSMCs的细胞计数、[3 H ″]TdR和[3 H]Leu掺入减少。与钙化组相比，早期治疗组VSMCs形态学改变延迟。提示牛磺酸可减轻血管平滑肌细胞的钙化。当治疗开始较早时，牛磺酸治疗似乎更有益。
Vascular calcification is an ectopic calcification that commonly occurs in atherosclerosis. Because taurine was previously shown to protect against cardiovascular diseases, the effect of taurine on vascular calcification was evaluated in calcified vascular smooth muscle cells (VSMCs) of rat in vitro in the present study. Osteoblastic differentiation, calcification, and proliferation in VSMCs were detected in the presence and absence of taurine. Alkaline phosphatase (ALP), cellular calcium content, and45Ca accumulation were measured as the indicators of osteoblastic differentiation and calcification. Incubation of VSMCs with Β-glycerophosphate for 10 days induced an osteoblast-like morphological change. The activity of ALP was enhanced. Calcium content and45Ca uptake were increased in these cells. Calcification of these VSMCs was demonstrated with Β-glycerophosphate treatment. In association with these alterations, cell proliferation, detected by cell counting, [3H]thymidine ([3H]TdR), and [3H]leucine ([3H]Leu) incorporation, was also increased in these calcified VSMCs. Taurine at 20 mmol/l decreased calcium content,45Ca2+uptake, and ALP activity both after early and late treatment, in which a reduction of the cell count, [3H″]TdR, and [3H]Leu incorporation of calcified VSMCs was also noted. Compared with the calcified group, morphological changes in the VSMCs of the early-treated group were deferred. These results demonstrated that calcification of VSMCs could be alleviated by taurine. Taurine treatment appeared to be more beneficial when the treatment was started earlier.