Urocortinergic neurons respond in a differentiated manner to various acute stressors in the Edinger-Westphal nucleus in the rat
Urocortinergic neurons respond in a differentiated manner to various acute stressors in the Edinger-Westphal nucleus in the rat
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DOI：
10.1002/cne.20343
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发表时间：
2004-12-06
影响因子：
2.5
通讯作者：
Kozicz, T
中科院分区：
文献类型：
--
作者：
Gaszner, B;Csernus, V;Kozicz, T
促肾上腺皮质激素释放因子（CRF）被认为是神经化学介导的应激反应中枢调节的主要贡献者；然而，越来越多的证据表明，除了 CRF 之外，该神经肽家族的其他成员，例如尿皮质素 (Ucn)，也可能在改变免疫、内分泌和对压力的行为反应的传出成分中发挥作用。 Ucn 在大鼠大脑中的分布已得到证实，最丰富的 Ucn 免疫反应性周核存在于 Edinger-Westphal 核 (E-WN)。急性疼痛和固定应激会招募 E-WN 神经元，然而，尚未在一项研究中比较 E-WN Ucn 神经元响应各种急性全身性和神经源性挑战的激活模式。因此，我们将定量 Fos 成像作为神经元激活的标记物与尿皮质素免疫组织化学相结合，以可视化由静脉内脂多糖（LPS；100 杯/千克）、乙醚吸入、约束、高渗（1.5 M 氯化钠腹腔注射）和低血压出血挑战诱导的神经元。 E-WN 中的神经元对 LPS 产生最强的 Fos 诱导反应，但乙醚和束缚应激也会在应激后 2 小时导致大量 Fos 免疫反应。出乎意料的是，高渗性和低血压性出血应激在应激后 2 小时并未在该大脑区域诱导尿皮质能核周。这种针对 E-WN 神经元的挑战特异性募集与应激诱导的肾上腺反应无关。我们将讨论 E-WN 尿皮质能神经元的生物学意义和应激特异性激活。 (C) 2004 Wiley-Liss, Inc.
Corticotropin-releasing factor (CRF) was implicated as being a major contributor to the neurochemically mediated central regulation of stress response; however, an increasing body of evidence suggests that, besides CRF, other members of this neuropeptide family, such as urocortin (Ucn), may also play a role in modifying the efferent components of immune, endocrine, and behavioral responses to stress. Ucn's distribution in the rat brain has been demonstrated, with the most abundant Ucn-immunoreactive perikarya present in the Edinger-Westphal nucleus (E-WN). Acute pain and immobilization stresses recruit E-WN neurons, however, the activation pattern of E-WN Ucn neurons in response to various acute systemic and neurogenic challenges has not been compared in a single study. We therefore combined quantitative Fos imaging as a marker for neuronal activation with urocortin immunohistochemistry to visualize neurons induced by intravenous lipopolysaccharide (LPS; 100 mug/kg), ether inhalation, restraint, hyperosmotic (1.5 M NaCl i.p.), and hypotensive hemorrhage challenges. Neurons in the E-WN responded with the strongest Fos induction to LPS, but ether and restraint stress also resulted in massive Fos immunoreactivity 2 hours after stress. Unexpectedly, hyperosmotic and hypotensive hemorrhage stresses did not induce urocortinergic perikarya in this brain area 2 hours poststress. This challenge-specific recruitment of E-WN neurons was independent of stress-induced adrenal response. The biological significance and the stress-specific activation of E-WN urocortinergic neurons will be discussed. (C) 2004 Wiley-Liss, Inc.