A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
复制标题
改善小鼠胆道闭锁综合征的银纳米颗粒方法。
DOI：
10.3791/58158
复制
发表时间：
2018-10-01
影响因子：
1.2
通讯作者：
Zhang, Ruizhong
中科院分区：
文献类型：
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作者：
Fu, Ming;Lin, Zefeng;Zhang, Ruizhong
胆道闭锁是一种严重的儿童胆管炎，死亡率高，其病因尚未完全清楚。病毒感染可能是一个可能的原因。用于研究BA的典型动物模型是通过用恒河猴轮状病毒感染新生小鼠来建立的。银纳米颗粒已被证明发挥抗菌和抗病毒作用，其功能在BA小鼠模型中进行了评估，在这项研究中。目前，在BA动物实验中，用于改善BA小鼠症状的方法通常是通过食物或其他药物给予对症治疗。本研究的目的是证明一种通过腹腔注射银纳米颗粒改善小鼠BA综合征的新方法，并提供制备银纳米颗粒凝胶制剂的详细方法。该方法简便易行，适用范围广，可用于BA作用机制的研究及临床治疗。基于BA小鼠模型，当小鼠表现出黄疸时，将制备的银纳米颗粒凝胶腹膜内注射到肝脏下部的表面。观察小鼠存活情况，并进行生化指标和肝脏组织病理学检查。这种方法允许更直观地理解BA模型的建立和新的BA治疗。
Biliary atresia (BA) is a severe type of cholangitis with high mortality in children of which the etiology is still not fully understood. Viral infections may be one possible cause. The typical animal model used for studying BA is established by inoculating a neonatal mouse with a rhesus rotavirus. Silver nanoparticles have been shown to exert antibacterial and antiviral effects; their function in the BA mouse model is evaluated in this study. Currently, in BA animal experiments, the methods used to improve the symptoms of BA mice are generally symptomatic treatments given via food or other drugs. The aim of this study is to demonstrate a new method for ameliorating BA syndrome in mice by the intraperitoneal injection of silver nanoparticles and to provide detailed methods for preparing the silver nanoparticle gel formulation. This method is simple and widely applicable and can be used to research the mechanism of BA, as well as in clinical treatments. Based on the BA mouse model, when the mice exhibit jaundice, the prepared silver nanoparticle gel is injected intraperitoneally to the surface of the lower liver. The survival status is observed, and biochemical indicators and liver histopathology are examined. This method allows a more intuitive understanding of both the establishment of the BA model and novel BA treatments.