HEAT-SHOCK PROTEINS, 1ST MAJOR PRODUCTS OF ZYGOTIC GENE ACTIVITY IN MOUSE EMBRYO
HEAT-SHOCK PROTEINS, 1ST MAJOR PRODUCTS OF ZYGOTIC GENE ACTIVITY IN MOUSE EMBRYO
复制标题
DOI：
10.1038/305331a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
JACOB, F
中科院分区：
文献类型：
--
作者：
BENSAUDE, O;BABINET, C;JACOB, F
在许多物种中，受精后早期的卵子发育似乎主要发生在母体控制下，不需要胚胎基因组的转录。在小鼠中，这种情况仅限于单细胞阶段;胚胎基因组的激活发生在两细胞阶段的晚期2 - 4，并导致合成蛋白质谱的急剧变化。在这种激活之前，在单细胞阶段结束时，蛋白质的总合成减少5 - 7，在双细胞阶段早期，出现了一组分子量为70，000（70 K）的新多肽（参考文献2，8，9）。这可以与分化细胞高温后发生的一系列事件进行比较10 -13。热休克导致大多数转录和翻译的停滞;随后，有限的一组基因（热休克基因）的表达先于细胞基因组的整体重新激活。在这里，我们表明，70 K早期的两个细胞特异性蛋白是相同的两个小鼠热休克蛋白，HSP 68和HSP 70。
In many species, the early post-fertilization development of the egg appears to occur mainly under maternal control and does not require transcription of the embryonic genome1. In the mouse this situation is restricted to the one-cell stage; activation of the embryonic genome occurs at the late two-cell stage2–4and results in a drastic change in the spectrum of proteins synthesized. This activation is preceded by a decrease in the overall synthesis of proteins at the end of the one-cell stage5–7and the appearance, at the early two-cell stage, of a set of new polypeptides of molecular weight ∼70,000 (70K) (refs 2, 8, 9). This can be compared with the series of events that occur after hyperthermia in differentiated cells10–13. Heat shock results in an arrest of most transcription and translation; subsequently, expression of a limited set of genes, the heat shock genes, precedes the overall reactivation of cellular genome. Here we show that the 70K early two-cell-specific proteins are identical to two of the mouse heat shock proteins, HSP 68 and HSP 70.