Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses
Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses
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DOI：
10.1016/j.enzmictec.2010.09.014
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发表时间：
2011-01-05
影响因子：
3.4
通讯作者：
Zhu, J. Y.
中科院分区：
文献类型：
--
作者：
Luo, Xiaolin;Zhu, J. Y.
研究了干燥过程中纤维角质化对木质纤维素底物酶糖化的影响。使用两种化学预处理的木材基材和一种商业漂白的硫酸盐硬木纸浆。在105和150 ° C下进行热干燥以及在50%RH和23.8 ° C下进行不同持续时间的空气干燥，以产生具有各种程度的角质化的基底。结果发现，从相同的从未干燥的样品制成的激素底物的底物酶消化率（SED）非常好地关联到一个容易测量的参数，保水值（WRV），并可以通过玻尔兹曼函数拟合。在给定的角质化程度下，当基质完全角质化时，角质化产生的SED减少量占总SED减少量的百分比取决于两个参数。第一个是WRV，其主要是有效酶分子大小的函数，和Delta，其与底物孔径分布形状有关。低值的SEDCH，SED的一个完全hormonic基板，从曲线拟合的三组样品的研究，表明酶的纤维素的可及性主要是通过细胞壁中的孔，而不是基板的外表面。发现激素底物的SED与Simons染色测量良好相关。提出了一个新的参数，以更好地关联酶的可及性纤维素使用双色西蒙斯染色技术。爱思唯尔公司出版
This study investigated the effect of fiber hornification during drying on lignocellulosic substrate enzymatic saccharification. Two chemically pretreated wood substrates and one commercial bleached kraft hardwood pulp were used. Heat drying at 105 and 150 degrees C and air drying at 50% RH and 23.8 degrees C for different durations were applied to produce substrate with various degrees of hornification. It was found that substrate enzymatic digestibilities (SEDs) of hornified substrates made from the same never-dried sample correlate very well to an easily measurable parameter, water retention value (WRV), and can be fitted by a Boltzmann function. The hornification-produced SED reduction at a given degree of hornification as the percentage of the total SED reduction when the substrate is completely hornified depends on two parameters. The first is WRV, which is primarily a function of the effective enzyme molecule size, and Delta, which is related to the substrate pore size distribution shape. The low values of SEDCH, SED of a completely hornified substrate, obtained from curve fittings for the three sets of samples studied, suggest that enzyme accessibility to cellulose is mainly through the pores in the cell wall rather than substrate external surface. The SEDs of hornified substrates were found to correlate to Simons' staining measurements well. A new parameter was proposed to better correlate enzyme accessibility to cellulose using the two-color Simons' staining technique. Published by Elsevier Inc.