Expression of mitochondria-related genes in lymphoblastoid cells from patients with bipolar disorder
Expression of mitochondria-related genes in lymphoblastoid cells from patients with bipolar disorder
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DOI：
10.1111/j.1399-5618.2005.00184.x
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发表时间：
2005-04-01
影响因子：
5.4
通讯作者：
Kato, T
中科院分区：
文献类型：
--
作者：
Washizuka, S;Kakiuchi, C;Kato, T
目的：多项研究表明双相情感障碍患者存在线粒体异常。我们报道了位于18p11的线粒体复合体I亚单位基因NDUFV2与双相情感障碍的关系。与对照组相比，双相情感障碍患者中该基因的mRNA表达水平降低。然而，目前还不清楚是否只有NDUFV2基因在双相情感障碍中表现出表达水平的降低。本研究旨在阐明其他核编码的复合体I亚单位基因和线粒体相关基因与双相情感障碍的关系。方法：采用实时定量逆转录聚合酶链式反应方法，对21例双相情感障碍患者和11名对照的淋巴母细胞系中除NDUFV2外的5个线粒体复合体I亚基基因、3个复合体IV亚单位基因和4个线粒体转录相关基因的mRNA表达水平进行了定量研究。此外，这些基因的表达水平降低与NDUFV2的表达水平相关。结论：NDUFV2基因的表达降低对线粒体呼吸链中的其他亚单位基因有相当大的影响，进一步证实了NDUFV2在双相情感障碍中的生物学意义。
Objectives: Several studies have suggested mitochondrial abnormality in bipolar disorder. We reported the association of mitochondrial complex I subunit gene, NDUFV2 at 18p11, with bipolar disorder. A decrease in the mRNA expression of this gene was found in patients with bipolar disorder compared with controls. However, it was unclear whether only the NDUFV2 gene exhibited the decreased expression level in bipolar disorder. The aim of this study was to clarify the association of other nuclear-encoded complex I subunit genes and mitochondria-related genes with bipolar disorder.Methods: We quantified the mRNA expression level of five nuclear-encoded mitochondrial complex I subunit genes located at the chromosomal regions linked with bipolar disorder other than NDUFV2, three complex IV subunit genes, and four mitochondrial transcription-related genes using a real-time quantitative reverse transcription polymerase chain reaction method in the lymphoblastoid cell lines from 21 patients with bipolar disorder and 11 controls.Results: Decreased mRNA expression in patients with bipolar I disorder compared with control subjects was found in most of the complex I subunit genes. In addition, decreased expression levels of these genes correlated with that of NDUFV2. No statistically significant alterations of mRNA expression levels were found between bipolar patients and controls among two of three complex IV subunit genes and all transcription-related genes.Conclusions: Our study suggests that the decreased expression of NDUFV2 has a considerable effect on other subunit genes in the mitochondrial respiratory chain and presents further evidence of the biological significance of NDUFV2 in bipolar disorder.