Effects of nicotine, its metabolites and tobacco extracts on human platelet function in vitro
Effects of nicotine, its metabolites and tobacco extracts on human platelet function in vitro
复制标题
DOI：
10.1016/j.tiv.2013.01.004
复制
发表时间：
2013-03-01
影响因子：
3.2
通讯作者：
Whiss, Per A.
中科院分区：
文献类型：
--
作者：
Ljungberg, Liza U.;Persson, Karin;Whiss, Per A.
吸烟是导致心血管疾病的主要原因。吸烟对心血管的影响可能是多因素的，包括对血小板的影响。以前关于尼古丁和烟草对血小板功能影响的研究报告并不一致。本研究在体外研究了尼古丁及其主要代谢物、烟草提取物和无烟草鼻烟提取物对人血小板的影响。代谢物可替宁、可替宁- n -氧化物、尼古丁-1′- n -氧化物和反式-3′-羟基可替宁（0.1-10 μ M）均不影响血小板聚集或p -选择素的表达。尼古丁（10 μ M）微弱地促进血小板聚集，而反式3′-羟基可替宁（0.1 μ M）和尼古丁-1′- n -氧化物（1-10 μ M）微弱地抑制纤维蛋白原粘附。为了阐明其他烟草成分对血小板功能的影响，我们研究了湿烟和烟提取物对血小板功能的影响。口服鼻烟、香烟烟雾和无烟草鼻烟的过滤提取物对血小板粘附的抑制呈浓度依赖性。烟草提取物对血小板粘附的抑制作用不依赖于尼古丁含量和一氧化氮途径，不通过血小板-尼古丁受体介导。综上所述，烟草提取物在短期体外刺激中抑制血小板活化。由于只观察到尼古丁和尼古丁代谢物的有限作用，烟草诱导的血小板抑制可能是由烟草和无烟草鼻烟中存在的其他化合物诱导的。(C) 2013 Elsevier Ltd.版权所有。
Cigarette smoking is a leading cause of cardiovascular disease. The cardiovascular effects of smoking are probably multifactorial, including effects on platelets. Previous reports investigating the effects of nicotine and tobacco on platelet function are inconsistent.The present study investigated in vitro effects of nicotine, its major metabolites, tobacco extracts and extract of tobacco-free snuff on human platelets.None of the metabolites cotinine, cotinine-N-oxide, nicotine-1'-N-oxide or trans-3'-hydroxycotinine (0.1-10 mu M) affected platelet aggregation or P-selectin expression. Nicotine (10 mu M) weakly increased platelet aggregation, whereas trans-3'-hydroxycotinine (0.1 mu M) and nicotine-1'-N-oxide (1-10 mu M) weakly inhibited adhesion to fibrinogen. To elucidate the influence of other tobacco compounds, we investigated the impact of moist tobacco and smoke extracts on platelet function. Filtered extracts of oral snuff, cigarette smoke and tobacco free snuff inhibited platelet adhesion concentration-dependently. The inhibitory effects of tobacco extracts on platelet adhesion were independent of nicotine content and the nitric-oxide-pathway and not mediated through a platelet-nicotine-receptor.Taken together, tobacco extracts inhibit platelet activation during short-term in vitro challenge. As only limited effects of nicotine and nicotine metabolites were seen, the tobacco-induced platelet inhibition are likely induced by other compounds present in tobacco and tobacco free snuff. (C) 2013 Elsevier Ltd. All rights reserved.