Usefulness of bronchoalveolar lavage for diagnosis of acute and persistent respiratory syncytial virus lung infections in guinea pigs
Usefulness of bronchoalveolar lavage for diagnosis of acute and persistent respiratory syncytial virus lung infections in guinea pigs
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DOI：
10.1002/(sici)1099-0496(199812)26:6
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发表时间：
1998-12-01
影响因子：
3.1
通讯作者：
Hegele, RG
中科院分区：
文献类型：
--
作者：
Dakhama, A;Chan, NG;Hegele, RG
为了研究支气管肺泡损伤 (BAL) 液体标本是否可用于诊断豚鼠急性和持续性呼吸道合胞病毒 (RSV) 肺部感染，我们测试了 BAL 液体和肺组织标本以获取病毒感染的证据，并比较了感染动物和未感染动物之间的 BAL 细胞学检查。对接种 RSV 的豚鼠在急性细支气管炎（接种后第 3 和 7 天）、恢复期（接种后第 14 天）和持续感染（接种后第 28 和 60 天）期间进行了研究；并与假感染的对照动物进行比较。对 BAL 和肺组织标本进行病毒培养，并通过免疫细胞化学检测病毒蛋白。采用逆转录聚合酶链反应（RT-PGR）方法检测病毒核酸。在每个研究日，比较接种 RSV 的动物和对照动物之间的总 BAL 细胞计数和差异 BAL 细胞计数。在 BAL 样本中，接种后第 3 天，四分之一的动物通过培养分离出复制的 RSV；接种后第 3-14 天，所有接触病毒的动物的 RSV 抗原免疫细胞化学呈阳性，并且在接种后第 3 天，四分之一的动物通过 RT-PCR 检测到病毒核酸。相比之下，在所有研究日从相同 RSV 感染动物获得的肺组织中都记录了复制病毒、病毒抗原和病毒核酸；与对照组相比，RSV 接种动物的 BAL 样本在所有研究日均含有更多的嗜酸性粒细胞（双尾 P 值 < 0.01）。这项动物研究的结果表明，BAL 液对于诊断持续性 RSV 感染没有用处。然而，当免疫细胞化学可以提供比 RT-PCR 或病毒培养更准确的病毒检测测试时，BAL 液可能有助于记录急性 RSV 肺部感染。小儿肺科。 1998； 26：396-404。 (C) 1998 Wiley-Liss, Inc.
To investigate whether bronchoalveolar ravage (BAL) fluid specimens can be used to diagnose acute and persistent respiratory syncytial virus(RSV) lung infections In guinea pigs, we tested BAL fluid and lung tissue specimens for evidence of viral infection, and compared BAL cytology between infected and uninfected animals. RSV-inoculated guinea pigs were studied during acute bronchiolitis (days 3 and 7 postinoculation), convalescence (Day 14 postinoculation), and persistent infection (Days 28 and 60 postinoculation); and were compared to the sham-infected control animals. BAL and lung tissue specimens were cultured for virus and tested by immunocytochemistry for viral protein. A reverse transcription polymerase chain reaction (RT-PGR) method was used to test for viral nucleic acid. Total and differential BAL cell counts were compared between RSV-inoculated and control animals on each study day.In BAL specimens, replicating RSV was isolated by culture in one out of four of the animals on Day 3 postinoculation; immunocytochemistry for RSV antigens was positive in all virus-exposed animals from Days 3-14 postinoculation, and viral nucleic acid was detected by RT-PCR in one-fourth of the animals on Day 3 postinoculation. In contrast, replicating virus, viral antigens, and viral nucleic acid were documented in lung tissues obtained from the same RSV-infected animals on all study days; BAL specimens of RSV-inoculated animals contained more eosinophils on all study days (two-tailed P value < 0.01) compared to the controls. The results of this animal study demonstrate that BAL fluid is not useful for diagnosis of persistent RSV infection. However, BAL fluid may be helpful for the documentation of acute RSV lung infection when immunocytochemistry may provide a more accurate test for virus detection than RT-PGR or viral culture. Pediatr Pulmonol. 1998; 26:396-404. (C) 1998 Wiley-Liss, Inc.