Activation of specific glutamate receptor subtypes increases c-fos proto-oncogene expression in primary cultures of neonatal rat cerebellar granule cells
Activation of specific glutamate receptor subtypes increases c-fos proto-oncogene expression in primary cultures of neonatal rat cerebellar granule cells
复制标题
特定谷氨酸受体亚型的激活增加新生大鼠小脑颗粒细胞原代培养物中c-fos原癌基因的表达
DOI：
10.1016/0028-3908(87)90132-8
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发表时间：
1987
影响因子：
4.7
通讯作者：
E. Costa
中科院分区：
文献类型：
--
作者：
A. Székely;M. Barbaccia;E. Costa
在原代培养的大鼠小脑颗粒细胞中，1-谷氨酸激活兴奋性氨基酸受体可增强c-fos原癌基因信使RNA的稳态水平。这种作用被镁（ImM）以及谷氨酸受体拮抗剂2-氨基-5-膦酰基-戊酸（APV）阻断。在其他兴奋性氨基酸激动剂N-甲基-D-天冬氨酸（NMDA）和使君子酸也增加了c-fos mRNA的含量，但后者在显着较小的程度，而红藻氨酸未能修改的基础水平的c-fos的表达。此外，毒蕈碱激动剂卡巴胆碱或抑制性神经递质GABA不影响c-fos mRNA的基础水平。该数据首次证明，激活特定兴奋性氨基酸受体亚型的信号转导可以增加原代培养的小脑神经元中c-fos原癌基因mRNA的稳态水平。
In primary cultures of rat cerebellar granule cells the activation of excitatory amino acid receptors by 1-glutamate enhances the steady state level of c-fos proto-oncogene messenger RNA. This effect is blocked by magnesium (ImM) as well as by the glutamate receptor antagonist 2-amino-5-phosphono-valerate (APV). Among the other excitatory amino acid agonists N-methyl-D-Aspartate (NMDA) and quisqualate also increased c-fos mRNA content, the latter however to a significantly lesser extent, while kainate failed to modify the basal level of c-fos expression. The addition of the muscarinic agonist carbachol or of the inhibitory neurotransmitter GABA did not affect the basal level of c-fos mRNA. This data demonstrate for the first time that activation of signal transduction at a specific excitatory amino acid receptor subtype can increase the steady state level of c-fos proto-oncogene mRNA in primary culture of cerebellar neurons.