The bacteriological composition of biomass recovered by flushing an operational drinking water distribution system
The bacteriological composition of biomass recovered by flushing an operational drinking water distribution system
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DOI：
10.1016/j.watres.2014.01.049
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发表时间：
2014-05-01
期刊：
影响因子：
12.8
通讯作者：
Boxall, J. B.
中科院分区：
文献类型：
--
作者：
Douterelo, I.;Husband, S.;Boxall, J. B.
本研究探讨了管道特性的影响，从饮用水分配系统（DWDS）和生物膜去除对水质的影响，在一个单一的英国分布管理区（DMA）与聚乙烯和铸铁管段的材料动员的细菌学组成的消防栓进行流量递增增加从管壁材料。在这些行动中监测了湍流，并收集了水样进行物理化学和细菌学分析。在冲洗之前和冲洗期间，从动员到大量水中的材料中提取DNA。然后用细菌标签编码的454焦磷酸测序来表征这种材料中存在的细菌群落。铁，铝，锰和磷酸盐的浓度被发现与观察到的浊度。从管道中动员的材料的细菌群落组成在塑料和铸铁管段之间有显著差异（p < 0.5）。从塑料管中去除的材料中检测到高相对丰度的α-变形菌（23.3%），梭菌（10.3%）和放线菌（10.3%）。从铸铁管中获得的样品中与α-变形菌（22.8%）、芽孢杆菌（16.6%）和γ-变形菌（1.4%）相关的序列占优势。最高的物种丰富度和多样性被发现在从塑料管动员的材料的样品。螺旋体属，甲基杆菌属梭菌属和脱硫杆菌属，是在冲洗塑料管之前和期间获得的材料中最具代表性的属。在铸铁管中，发现了能够利用不同铁和锰化合物的相对丰度高的细菌，例如赖氨酸芽孢杆菌属，土芽孢杆菌属和磁细菌属（Magneto-bacterium spp.）(C)2014爱思唯尔有限公司版权所有。
This study investigates the influence of pipe characteristics on the bacteriological composition of material mobilised from a drinking water distribution system (DWDS) and the impact of biofilm removal on water quality.Hydrants in a single UK Distribution Management Area (DMA) with both polyethylene and cast iron pipe sections were subjected to incremental increases in flow to mobilise material from the pipe walls. Turbidity was monitored during these operations and water samples were collected for physico-chemical and bacteriological analysis. DNA was extracted from the material mobilised into the bulk water before and during flushing. Bacterial tag-encoded 454 pyrosequencing was then used to characterize the bacterial communities present in this material.Turbidity values were high in the samples from cast iron pipes. Iron, aluminium, manganese and phosphate concentrations were found to correlate to observed turbidity. The bacterial community composition of the material mobilised from the pipes was significantly different between plastic and cast iron pipe sections (p < 0.5). High relative abundances of Alphaproteobacteria (23.3%), Clostridia (10.3%) and Actinobacteria (10.3%) were detected in the material removed from plastic pipes. Sequences related to Alphaproteobacteria (22.8%), Bacilli (16.6%), and Gammaproteobacteria (1.4%) were predominant in the samples obtained from cast iron pipes. The highest species richness and diversity were found in the samples from material mobilised from plastic pipes. Spirochaeta spp., Methylobacterium spp. Clostridium spp. and Desulfobacterium spp., were the most represented genera in the material obtained prior to and during the flushing of the plastic pipes. In cast iron pipes a high relative abundance of bacteria able to utilise different iron and manganese compounds were found such as Lysinibacillus spp., Geobacillus spp. and Magneto-bacterium spp. (C) 2014 Elsevier Ltd. All rights reserved.