VARIANT SPECIFIC EPITOPES OF GIARDIA-LAMBLIA
VARIANT SPECIFIC EPITOPES OF GIARDIA-LAMBLIA
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DOI：
10.1016/0166-6851(90)90120-b
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发表时间：
1990-08-01
影响因子：
1.5
通讯作者：
MERRITT, JW
中科院分区：
文献类型：
--
作者：
NASH, TE;CONRAD, JT;MERRITT, JW
蓝氏贾第鞭毛虫经历表面抗原变异。用4株抗WB或WB样贾第鞭毛虫变异株(mAb6E7、5c1和3F6)和GS/M(mAbG10/4)的表面反应单抗(MAb4)测定不同分离株和克隆的能力。28株分离株中，11株与单抗6E7、5C1和3F6有反应，6株与单抗3F6有反应，2株与单抗G10/4有反应，1株与单抗6E7有反应，8株经直接或间接免疫荧光检测无反应。来自不同分离株的新建立的克隆产生了少量与其亲本相似的反应性滋养体。在任何一个滋养体上只发现一个表位。与mAb6E7识别的表位编码探针杂交的Southern杂交结果显示，大多数分离株不能表达该抗原是由于缺乏该基因。对3株mAb6E7反应性克隆的表面抗原分析表明，mAb6E7可与不同分子质量的表面抗原发生反应。
Giardia lamblia undergoes surface antigenic variation. The capability of different isolates and clones was determined using 4 surface-reacting monoclonal antibodies (mAbs) to variants derived from WB or WB-like Giardia (mAbs 6E7, 5C1 and 3F6) and GS/M (mAB G10/4). Of 28 isolates, 11 possessed trophozoites reactive with mAbs 6E7, 5C1 and 3F6, 6 with mAb 3F6, 2 with Mab G10/4, 1 with mAb 6E7, and 8 showed no reactivity as determined by direct or indirect immunofluorescence. Newly established clones from different isolates generated small numbers of reactive trophozoites similar to their parents. Only one epitope was found on any single trophozoite. Southern blots hybridized to a probe encoding for the epitope recognized by mAb 6E7 revealed that the inability to express the antigen in most isolates was due to lack of the gene. Analysis of the surface antigens of mAb 6E7 reactive clones from 3 isolates revealed that mAb 6E7 reacted with surface antigens of different molecular masses.