Effect of UV-irradiation on DNA replication of the parvovirus minute-virus-of-mice in mouse fibroblasts.
Effect of UV-irradiation on DNA replication of the parvovirus minute-virus-of-mice in mouse fibroblasts.
复制标题
紫外线照射对小鼠成纤维细胞中细小病毒-小鼠微小病毒 DNA 复制的影响。
DOI：
10.1093/nar/10.8.2577
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发表时间：
1982
影响因子：
14.9
通讯作者：
Ward,DC
中科院分区：
文献类型：
--
作者：
Rommelaere,J;Ward,DC
研究了紫外线照射对小鼠细小病毒（MVM）单链DNA感染小鼠A9成纤维细胞后转化为双链复制型（RF）的影响。在感染未照射的细胞之前，病毒的UV照射导致RF形成的剂量依赖性、单次命中抑制。限制性片段分析表明，这种抑制作用可以归因于引入绝对块，防止新合成的互补链的延伸。在用UV照射的MVM感染之前，细胞暴露于UV光增强了转化为RF的输入病毒DNA的分数。这种增强需要在细胞照射和病毒感染之间的间隔期间合成新蛋白。这些结果表明，DNA复制构成了病毒生命周期中的一个靶点，导致UV增强的病毒存活再激活，然而，它们不允许我们鉴定在UV预处理的细胞中增强的RF形成步骤。
The effect of UV-irradiation on the conversion of the single-stranded DNA of the parvovirus Minute-Virus-of-Mice (MVM) to duplex Replicative Forms (RF) was studied after infection of mouse A9 fibroblasts. UV-irradiation of the virus prior to infection of unirradiated cells resulted in a dose-dependent, single-hit, inhibition of RF formation. Restriction fragment analysis indicated that this inhibition could be ascribed to the introduction of absolute blocks which prevent elongation of the newly synthesized complementary strand. Cell exposure to UV-light prior to infection with UV-irradiated MVM enhanced the fraction of input viral DNA which was converted to RF. This enhancement requiredde novoprotein synthesis during the interval between cell irradiation and virus infection. These results suggest that DNA replication constitutes a target in the viral life cycle that leads to the UV-enhanced Reactivation of virus survival, however they do not permit us to identify the step of RF formation which is enhanced in UV-pretreated cells.
DOI：
10.1086/413070
发表时间：
1983
期刊：
The Quarterly Review of Biology
影响因子：
--
作者：
A. Anilionis
通讯作者：
A. Anilionis