Corrigendum to “Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II in Thermosynechococcus elongatus”
Corrigendum to “Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II in Thermosynechococcus elongatus”
复制标题
“D1 亚基的组氨酸 198 对热聚球藻光系统 II 的主要电子供体 P680 特性的影响”的勘误表
DOI：
10.1016/j.bbabio.2016.09.012
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and Boussac A.
中科院分区：
文献类型：
--
作者：
Sugiura;M.;Ozaki;Y.;Rappaport F.;and Boussac A.
先前已经在长形热聚球藻中构建了两个突变体D1-H198 Q和D1-H198 A，目的是通过改变PD 1的轴向配体来改变P680·+/P680对的氧化还原电位，PD 1是光系统II初级电子供体的两个叶绿素之一[Sugiura et al.，生物化学生物Biophys. Acta 1777（2008）331-342]。然而，在这项工作发表后，Eberhard Schlodder博士向我们指出，（Technische Universität柏林）在两种突变体中，在QA还原时观察到的脱镁叶绿素谱带位移集中在544 nm而不是547 nm，清楚地表明D1蛋白对应于PsbA 1，而突变体被认为是在psbA 3基因中构建的，因此我们先前的结论是错误的。纸是错的。对这些突变体的分析表明，它们是适合进一步研究的正确突变体。事实上，D1-H198 Q突变使Soret区的P680·+/P680差吸收光谱降低了约3 nm，并增加了P680·+/P680对的氧化还原电位，D1-H198 A突变降低了P680·+/P680对的氧化还原电位，所有这些效应与在集胞藻中观察到的效应相当。PCC 6803 [Diner等人，Biochemistry 40（2001）9265-9281和Merry等人Biochemistry 37（1998）17，439 - 17，447]。我们为在我们之前的出版物中提供错误的数据和错误的结论道歉。
Two mutants, D1-H198Q and D1-H198A, have been previously constructed inThermosynechococcus elongatuswith the aim at modifying the redox potential of the P680•+/P680couple by changing the axial ligand of PD1, one the two chlorophylls of the Photosystem II primary electron donor [Sugiura et al., Biochim. Biophys. Acta 1777 (2008) 331–342]. However, after the publication of this work it was pointed out to us by Dr. Eberhard Schlodder (Technische Universität Berlin) that in both mutants the pheophytin band shift which is observed upon the reduction of QAwas centered at 544 nm instead of 547 nm, clearly showing that the D1 protein corresponded to PsbA1 whereas the mutants were supposedly constructed in thepsbA3gene so that the conclusions in our previous paper were wrong. O2evolving mutants have been therefore reconstructed and their analyze shows that they are now correct mutants which are suitable for further studies. Indeed, the D1-H198Q mutation downshifted by ≈ 3 nm the P680•+/P680difference absorption spectrum in the Soret region and increased the redox potential of the P680•+/P680couple and the D1-H198A mutation decreased the redox potential of the P680•+/P680couple all these effects being comparable to those which were observed inSynechocystissp. PCC 6803 [Diner et al., Biochemistry 40 (2001) 9265–9281 and Merry et al. Biochemistry 37 (1998) 17,439–17,447]. We apologize for having presented wrong data and wrong conclusions in our earlier publication.