A METHOD TO PERFORM DIRECT TRANSCUTANEOUS INTRATHECAL INJECTION IN RATS
A METHOD TO PERFORM DIRECT TRANSCUTANEOUS INTRATHECAL INJECTION IN RATS
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DOI：
10.1016/1056-8719(94)90087-6
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发表时间：
1994-12-01
影响因子：
1.9
通讯作者：
ESCHALIER, A
中科院分区：
文献类型：
--
作者：
MESTRE, C;PELISSIER, T;ESCHALIER, A
本研究描述了一种在大鼠中进行直接鞘内注射（i.t）而不引入脊髓导管的方法。它易于在清醒的动物中使用，产生快速和可重复的结果，没有运动损伤的迹象。每次注射的质量都是通过观察注射引起的甩尾来保证的。注射10 μ L的亚甲基蓝可以很好地定位，沿脊髓扩散非常有限。结果表明，吗啡具有明显的抗感觉作用，纳洛酮阻断了单神经病变大鼠全身吗啡的作用。
This work describes a method for performing direct intrathecal (i.t.) injections in rats without introducing a spinal catheter. Its ease of use in awake animals yields rapid and reproducible results with no sign of motor impairment. The quality of each injection was ensured by the observation of an injection-induced tail-flick. A 10-mu L injection of methylene blue was well localized yielding a very limited diffusion along the spinal cord. The method was validated by demonstrating that morphine (i.t.) had a marked antinociceptive effect and that naloxone (i.t.) blocked the effect of systemic (s.c.) morphine in mononeuropathic rat.