Estimation of particle number by stereology: An update
Estimation of particle number by stereology: An update
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DOI：
10.1089/0894268042176292
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发表时间：
2004-09-01
期刊：
影响因子：
--
通讯作者：
Geiser, M
中科院分区：
文献类型：
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作者：
Cruz-Orive, LM;Geiser, M
近年来，体视学规定的取样设计已被证明非常有用，可在光显微镜水平上原位估计不同肺室中沉积颗粒或巨噬细胞的总数。采样方法是基于平行板，随后由disectors子采样。由此产生的数量估计是无偏的，无论组织收缩或肿胀，他们很容易适用于其他情况下（特别是在神经科学）。设计的几个变体是可用的，但是，虽然它们都产生相同的数字估计，其精度，和数学预测，不同的估计之间的变化，并受到理论上的改进。本文详细介绍了颗粒截留和清除的最新进展，并以一个真实的实例说明了有关的方法和公式。
Sampling designs dictated by stereology have proven very useful in recent years to estimate in situ the total number of deposited particles, or of macrophages, in different lung compartments at the light microscopical level. The sampling methods are based on parallel slabs which are subsequently subsampled by disectors. The resulting number estimators are unbiased irrespective of tissue shrinkage or swelling, and they are readily applicable in other contexts (notably in neuroscience). Several variants of the design are available, however, and, although they all yield the same numbed estimates, their precision, and the mathematical prediction of it, vary among the different estimators and are subjected to theoretical improvement. The present paper constitutes a detailed survey of the latest advances, and it illustrates methods and formulae alike by way of a real example stemming from an earlier study on particle retention and clearance.