Identification of the structural determinants for efficient glucose transport via segment swapping between two fungal glucose transporters
Identification of the structural determinants for efficient glucose transport via segment swapping between two fungal glucose transporters
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通过两种真菌葡萄糖转运蛋白之间的片段交换鉴定有效葡萄糖转运的结构决定因素
DOI：
10.1039/c7ra01095h
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发表时间：
2017-05
期刊：
影响因子：
3.9
通讯作者：
Liu Weifeng
中科院分区：
文献类型：
--
作者：
Zhang Weixin;Cao Yanli;Chen Guanjun;Liu Weifeng
葡萄糖转运蛋白介导细胞内葡萄糖的摄取，并在葡萄糖代谢和信号转导中发挥重要作用。然而，我们对各种葡萄糖转运蛋白的结构基础的了解仍然有限。在这项研究中，我们发现从丝状真菌粗毛脉孢菌获得的葡萄糖转运蛋白HGT-1比从里氏木霉获得的其他糖转运蛋白Stp1具有更高的葡萄糖转运蛋白活性，尽管它们的序列同源性高达75.9%。为了确定Stp1和HGT-1所显示的差异葡萄糖转运能力的结构决定因素，通过用相应的HGT-1序列替换Stp1的特定序列区域来进行片段交换。对嵌合转运体的葡萄糖转运能力的研究表明，用HGT-1取代位于细胞内侧的Stp1的N-末端或C-末端片段可以明显提高其转运能力。这两个段的组合互换进一步将STP1的传输能力提高到几乎与HGT-1相当的水平。然而，C-端32个残基的缺失取消了Stp1的转运能力，而C-端16个残基的缺失明显提高了Stp1的转运能力。这些结果为深入了解细胞内葡萄糖转运蛋白的N-末端和C-末端在葡萄糖运输中的作用提供了线索。
Glucose transporters mediate the intracellular uptake of glucose and play important roles in glucose metabolism and signaling. Our understanding of the structural basis for a variety of glucose transporters is, however, still limited. In this study, we presented that the glucose transporter Hgt-1, obtained from the filamentous fungus Neurospora crassa, exhibited much higher glucose transport activity than the other sugar transporter Stp1, obtained from Trichoderma reesei, although they shared high sequence identity of 75.9%. To identify the structural determinants for differential glucose transport capability displayed by Stp1 and Hgt-1, segment swapping was performed by replacing specific sequence regions of Stp1 with the corresponding sequences of Hgt-1. Investigation of the glucose transport capability of the resulting chimeric transporters revealed that replacement of a N- or a C-terminal segment of Stp1, located on the intracellular side, with that of Hgt-1 can apparently improve its transport capability. The combined swapping of these two segments further enhanced the transport capability of Stp1 to a level almost comparable to that of Hgt-1. However, deletion of the C-terminal 32 residues abolished the transport capability of Stp1, whereas deletion of the C-terminal 16 residues apparently improved its transport capability. These results provide insight into the roles of intracellular N- and C-termini of glucose transporters in glucose transport.
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影响因子：
64.8
作者：
Nomura N;Verdon G;Kang HJ;Shimamura T;Nomura Y;Sonoda Y;Hussien SA;Qureshi AA;Coincon M;Sato Y;Abe H;Nakada-Nakura Y;Hino T;Arakawa T;Kusano-Arai O;Iwanari H;Murata T;Kobayashi T;Hamakubo T;Kasahara M;Iwata S;Drew D
通讯作者：
Drew D
影响因子：
64.5
作者：
Latorraca NR;Fastman NM;Venkatakrishnan AJ;Frommer WB;Dror RO;Feng L
通讯作者：
Feng L
影响因子：
12.9
作者：
Sabire Özcan;M. Johnston
通讯作者：
Sabire Özcan;M. Johnston
DOI：
10.1074/jbc.273.44.29106
发表时间：
1998-10
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
M. Kasahara;M. Maeda
通讯作者：
M. Kasahara;M. Maeda
影响因子：
4.1
作者：
Kasahara, T;Kasahara, M
通讯作者：
Kasahara, M