Experimental study of therapy of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel for the treatment of stress urinary incontinence
Experimental study of therapy of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel for the treatment of stress urinary incontinence
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DOI：
10.1002/nau.22291
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发表时间：
2013-03-01
影响因子：
2
通讯作者：
Xu, Jian-Wei
中科院分区：
文献类型：
--
作者：
Du, Xiao-Wen;Wu, Hui-Ling;Xu, Jian-Wei
目的探讨骨髓间充质干细胞或肌样细胞/海藻酸钙复合凝胶注射疗法对SUI大鼠模型尿道周围成肌细胞形成、尿道阻力增加及对LPP的影响。方法对SD大鼠骨髓间充质干细胞进行分离、培养和鉴定。引入 5-氮胞苷来诱导肌肉样细胞。在72只6周龄雌性SpragueDawley大鼠中产生SUI，将其分为四组：干细胞-凝胶组、肌肉样细胞-凝胶组、凝胶组和模拟对照组。注射后 1、4 和 8 周，测量泄漏点压力 (LPP)。对结蛋白和-骨骼肌肌动蛋白（-SMA）进行HE染色。结果 干细胞凝胶组和肌肉样细胞凝胶组注射后4、8周，凝胶边缘血管生长逐渐增多，荧光示踪剂观察新生血管周围有BMSC和肌肉样细胞聚集，肌肉样细胞长成细长的梭形细胞，Desmin、-SMA染色均明显阳性表达。模拟对照组的LPP测定与凝胶组相比有显着差异。结论 BMSC、肌肉样细胞和海藻酸钙复合凝胶复合物在SUI大鼠模型微环境中具有分化为肌肉细胞的潜力。通过LPP测量发现，尿道阻力的增加与海藻酸钙凝胶的体积效应相关性较高。神经尿素。尿动力学。 32: 281286, 2013。(c) 2012 Wiley 期刊公司。
Objective To explore the myoblast formation around the urethra and increase in urethral resistance of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel injection therapy and effect on LPP in SUI rat model. Methods Isolation, cultivation, and identification of SD rat bone marrow mesenchymal stem cell were performed. 5-Azacytidine was introduced to induce muscle-like cells. SUI was produced in 72 6-week-old female SpragueDawley rats, which were divided into four groups: stem cell-gel group, muscle-like cell-gel group, Gel group, and mock control group. One, 4, and 8 weeks after injection, the leak point pressure (LPP) was measured. HE staining of Desmin and -skeletal muscle actin (-SMA) were performed. Results At 4 and 8 weeks after injection in stem cell-gel group and muscle-like cell-gel group, growth of blood vessels gradually increased at gel edge, BMSC, and muscle-like cells gathered around the new blood vessels observed by fluorescence tracer, muscle-like cells grew into elongated spindle-like cells, Desmin, and -SMA staining were obviously positive expression. LPP determinations of the mock control group compared with the Gel groups were significantly different. Conclusions Compound of BMSC, muscle-like cells, and calcium alginate composite gel has the potential to differentiate into muscle cells in the microenvironment of SUI rat model. It is found by LPP measurement that the correlation between the increase in urethral resistance and the volume effect of calcium alginate gel is high. Neurourol. Urodynam. 32: 281286, 2013. (c) 2012 Wiley Periodicals, Inc.