Studying DNA Replication Fork Stability in Xenopus Egg Extract
Studying DNA Replication Fork Stability in Xenopus Egg Extract
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DOI：
10.1007/978-1-61779-129-1_25
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Costanzo, Vincenzo
中科院分区：
文献类型：
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作者：
Hashimoto, Yoshitami;Costanzo, Vincenzo
确保细胞存活和基因组稳定的一个关键过程是基因组的正确复制。DNA复制依赖于复杂的机制，其机制正在使用不同的模型系统来阐明。这个过程的一个主要方面是当复制叉子遇到阻碍它们前进的障碍时会发生什么，这是一个密集的研究主题，例如修改的碱基、暂停的位点和单链断裂。在DNA损伤存在的情况下，DNA复制的详细生化分析因缺乏概括DNA复制的无细胞系统而受到阻碍。在这里，我们描述了基于脊椎动物非洲爪哇卵提取液的分析，以研究复制叉稳定性的生化方面。
A crucial process to ensure cell survival and genome stability is the correct replication of the genome. DNA replication relies on complex machinery whose mechanisms are being elucidated using different model systems. A major aspect of this process, which is an intense subject of investigation, is what happens when replication forks encounter obstacles impairing their progression such as modified bases, pausing sites, and single strand breaks. The detailed biochemical analysis of DNA replication in the presence of DNA damage has been impeded by the lack of a cell-free system recapitulating DNA replication. Here we describe assays based on the vertebrate Xenopus laevis egg extract to study the biochemical aspects of replication fork stability.