Cloning, expression, and catalytic activity of Helicobacter hepaticus urease
Cloning, expression, and catalytic activity of Helicobacter hepaticus urease
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DOI：
10.1128/iai.69.9.5914-5920.2001
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发表时间：
2001-09-01
影响因子：
3.1
通讯作者：
Riley, LK
中科院分区：
文献类型：
--
作者：
Beckwith, CS;McGee, DJ;Riley, LK
肝螺杆菌引起小鼠肝脏和下肠道疾病。它是强烈的尿素酶阳性，虽然它不生活在酸性环境中。螺杆肝细胞尿素酶基因簇在有和没有幽门螺杆菌镍转运蛋白NixA共表达的大肠杆菌中表达。至于H. pylori中，很难从重组H. hepaticus尿素酶;需要包括NiCl 2补充的特殊条件。螺杆hepaticus尿素酶簇包含H. pylori尿素酶簇，包括尿素转运蛋白基因ureI。下游基因是大肠杆菌nik镍转运操纵子的同源基因。杆菌非胃H. hepaticus产生与H.幽门。
Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene cluster was expressed in Escherichia coli with and without coexpression of the Helicobacter pylori nickel transporter NixA. As for H. pylori, it was difficult to obtain enzymatic activity from recombinant H. hepaticus urease; special conditions including NiCl2 supplementation were required. The H. hepaticus urease cluster contains a homolog of each gene in the H. pylori urease cluster, including the urea transporter gene ureI. Downstream genes were homologs of the nik nickel transport operon of E. coli. Nongastric H. hepaticus produces urease similar to that of H. pylori.