Spontaneous mutations in diploid Saccharomyces cerevisiae:: More beneficial than expected
Spontaneous mutations in diploid Saccharomyces cerevisiae:: More beneficial than expected
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DOI：
10.1534/genetics.104.033761
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发表时间：
2004-12-01
期刊：
影响因子：
3.3
通讯作者：
Hall, DW
中科院分区：
文献类型：
--
作者：
Joseph, SB;Hall, DW
我们进行了1012代突变积累（MA）的酵母，酿酒酵母的实验。MA线表现出显着减少，平均健身和健身方差显着增加。我们发现，5.75%的适应性改变突变是有益的。这一发现与人们普遍认为的几乎所有改变健康的突变都是有害的观点相矛盾。突变率估计为每代每个单倍体基因组6.3 × 10 ~（-3）个突变，突变的平均杂合适合度效应为0.061。这些估计是兼容的酵母菌与以前的估计。
We performed a 1012-generation mutation-accumulation (MA) experiment in the yeast, Saccharomyces cerevisiae. The MA lines exhibited a significant reduction in mean fitness and a significant increase in variance in fitness. We found that 5.75% of the fitness-altering mutations accumulated were beneficial. This finding contradicts the widely held belief that nearly all fitness-altering mutations are deleterious. The mutation rate was estimated as 6.3 X 10(-3) mutations per haploid genome per generation and the average heterozygous fitness effect of a mutation as 0.061. These estimates are compatible with previous estimates in yeast.