WAP1, a wheat APETALA1 homolog, plays a central role in the phase transition from vegetative to reproductive growth
WAP1, a wheat APETALA1 homolog, plays a central role in the phase transition from vegetative to reproductive growth
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DOI：
10.1093/pcp/pcg171
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发表时间：
2003-12-01
影响因子：
4.9
通讯作者：
Ogihara, Y
中科院分区：
文献类型：
--
作者：
Murai, K;Miyamae, M;Ogihara, Y
小麦抽穗期的研究春化要求、光周期敏感性和狭义早熟性是决定水稻从营养生长向生殖生长转变的三个重要因素。我们鉴定并鉴定了小麦中的APETALA 1（AP 1）-like MADS box基因（WAP 1）作为相变激活剂。它的表达在相变之前开始，并在生殖阶段维持。通过共抑制抑制WAP 1的表达，既不影响春化要求，也不影响光周期敏感性，但导致延迟狭义早熟，表明WAP 1加速自主相变。对春性基因（Vrn）近等基因系（NILs）中WAP 1表达的分析表明，在Vrn显性基因的NILs中，春化强烈诱导WAP 1的表达，而在Vrn隐性基因的NILs中，春化弱诱导WAP 1的表达。此外，WAP 1的表达上调了长的光周期在两个近等基因组和那些没有光周期不敏感基因（Ppd）。这些结果表明，WAP 1基因是小麦营养生长向生殖生长转变调控途径中的一个关键基因。
Heading time in bread wheat (Triticum aestivum L.) is determined by three characters: vernalization requirement, photoperiodic sensitivity and narrow-sense earliness, which are involved in the phase transition from vegetative to reproductive growth. We identified and characterized the APETALA1 (AP1)-like MADS box gene in wheat (WAP1) as an activator of phase transition. Its expression starts just before the phase transition and is maintained during the reproductive phase. Inhibition of WAP1 expression in the transgenic plants by co-suppression affected neither vernalization requirement nor photoperiodic sensitivity, but resulted in delayed narrow-sense earliness, indicating that WAP1 accelerates autonomous phase transition. Analyses of the WAP1 expression in the near-isogenic lines (NILs) for spring habit genes (Vrn) revealed that WAP1 transcripts were induced by vernalization strongly in the NILs with Vrn dominant alleles and weakly with the recessive alleles. Furthermore, WAP1 expression was up-regulated by a long photoperiod in both NILs with and those without a photoperiod-insensitive gene (Ppd). These results suggest that WAP1 is a key gene in the regulatory pathway controlling the phase transition from vegetative to reproductive growth in wheat.