Highly Efficient Oxidation of Amines to Aldehydes with Flow-based Biocatalysis
Highly Efficient Oxidation of Amines to Aldehydes with Flow-based Biocatalysis
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DOI：
10.1002/cctc.201701147
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发表时间：
2017-10-23
期刊：
影响因子：
4.5
通讯作者：
Paradisi, Francesca
中科院分区：
文献类型：
--
作者：
Contente, Martina L.;Dall'Oglio, Federica;Paradisi, Francesca
以固定化纯转氨酶填充床反应器为基础，采用连续流动法，开发了一种温和、高效的醛类水溶液制备新工艺。HEWT是来自耐盐细菌长盐单胞菌的一种转氨酶，因其优异的稳定性和底物范围而被选择。用1 ~ 5当量丙酮酸钠，可将16种不同的胺迅速（3- 15分钟）氧化成相应的醛（90 ~ 99%）。该方法是完全自动化的，允许在线回收纯醛（化学纯度>99%，分离产率高于80%），而无需任何进一步的后处理程序。
A new mild and efficient process for the aqueous preparation of aldehydes, which are employed as flavour and fragrance components in food, beverage, cosmetics, as well as in pharmaceuticals, was developed using a continuous-flow approach based on an immobilised pure transaminase-packed bed reactor. HEWT, an -transaminase from the haloadapted bacterium Halomonas elongata, has been selected for its excellent stability and substrate scope. Sixteen different amines were rapidly (3-15min) oxidised to the corresponding aldehydes (90 to 99%) with only 1 to 5equivalents of sodium pyruvate. The process was fully automated, allowing for the in-line recovery of the pure aldehydes (chemical purity >99% and isolated yields above 80%), without any further work-up procedure.