Measuring the damage of heavy metal cadmium in rice seedlings by SRAP analysis combined with physiological and biochemical parameters.
Measuring the damage of heavy metal cadmium in rice seedlings by SRAP analysis combined with physiological and biochemical parameters.
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DOI：
10.1002/jsfa.6949
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发表时间：
2015-08
影响因子：
4.1
通讯作者：
Xiao-qin Zhang;Huinan Chen;Hua Jiang;Wenyi Lu;Jiangjie Pan;Q. Qian;Dawei Xue
中科院分区：
文献类型：
--
作者：
Xiao-qin Zhang;Huinan Chen;Hua Jiang;Wenyi Lu;Jiangjie Pan;Q. Qian;Dawei Xue
镉（Cd）是毒性最大的污染物之一，镉污染已成为制约水稻生产和品质提高的制约因素。因此，利用生物标志物对水稻镉污染进行监测具有重要意义。本研究采用序列相关扩增多态性（SRAP）和生理生化方法研究了镉胁迫对水稻的毒理学效应。结果随着Cd浓度和处理时间的增加，W7和M63两个水稻品种的叶绿素含量降低，丙二醛含量升高。这种趋势在1963年更为明显。抗氧化酶超氧化物歧化酶和过氧化物酶均显著高于对照组。SRAP聚合酶链反应结果表明，Cd处理与对照在SRAP谱和基因型上存在显著差异。基因组模板稳定性（GTS）随Cd浓度和持续时间的增加而降低。在相同处理条件下，W7的GTS高于M63。对比分析表明，Cd胁迫下水稻幼苗生理生化参数的变化与SRAP谱的变化具有良好的相关性。此外，SRAP谱的变化在水稻幼苗根系中表现出增强的敏感性。结论SRAP谱和生理生化参数可作为水稻生产过程中镉污染的生物标志物。
BACKGROUND Cadmium (Cd) is one of the most poisonous pollutants, and Cd pollution has become the limiting factor of rice production and quality improvement. Therefore it is of significant importance to monitor Cd toxicity by the detection of Cd contamination in rice with biomarkers. In the present study, sequence-related amplified polymorphism (SRAP) and physiological and biochemical methods were applied to determine the toxicological effects of Cd stress on rice. RESULTS With increasing Cd concentration and duration, the content of chlorophyll in the two rice varieties W7 and M63 decreased and that of malondialdehyde increased. This tendency was more apparent in M63. The antioxidant enzymes superoxide dismutase and peroxidase both increased significantly compared with controls. SRAP polymerase chain reaction results indicated significant differences between Cd treatments and controls in terms of SRAP profile, as well as genotypic differences. The genomic template stability (GTS) decreased with increasing Cd concentration and duration. Under the same treatment conditions, the GTS of W7 was higher than that of M63. Comparison analysis revealed that the changes in physiological and biochemical parameters of rice seedlings under Cd stress had a good correlation with the changes in SRAP profile. Furthermore, the changes in SRAP profile showed enhanced sensitivity in the roots of rice seedlings. CONCLUSION The SRAP profile and physiological and biochemical parameters could act as appropriate biomarkers for the measurement of Cd contamination during rice production.