A new surface plasmon resonance sensor for high-throughput screening applications
A new surface plasmon resonance sensor for high-throughput screening applications
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DOI：
10.1016/j.bios.2004.09.025
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发表时间：
2005-04-15
影响因子：
12.6
通讯作者：
Homola, J
中科院分区：
文献类型：
--
作者：
Piliarik, M;Vaisocherová, H;Homola, J
我们报道了一种新的高通量表面等离子体共振（SPR）传感器的基础上结合SPR成像与偏振对比和空间图案化的多层SPR结构。我们证明，这种方法提供了许多有利的功能，包括高对比度的SPR图像适合自动化计算机分析，相邻的传感通道之间的串扰最小和固有的光水平波动补偿。描述了具有108个传感通道的高通量SPR传感器的实验室原型用于折射和生物传感的应用。在折射率实验中，系统的噪声限制折射率分辨率已被确定为3 × 10 ~（-6）折射率单位（RIU）。检测人绒毛膜促性腺激素（hCG）的实验数据表明，与抗hCG的单克隆抗体结合，所报道的SPR成像传感器能够检测浓度低于500 ng/ml的hCG。(c)2004 Elsevier B. V.保留所有权利。
We report a new high-throughput surface plasmon resonance (SPR) sensor based on combination of SPR imaging with polarization contrast and a spatially patterned multilayer SPR structure. We demonstrate that this approach offers numerous advantageous features including high-contrast SPR images suitable for automated computer analysis, minimum crosstalk between neighboring sensing channels and inherent compensation for light level fluctuations. Applications of a laboratory prototype of the high-throughput SPR sensor with 108 sensing channels for refractometry and biosensing are described. In refractometric experiments, the noise-limited refractive index resolution of the system has been established to be 3 x 10(-6) refractive index unit (RIU). Experimental data on detection of human choriogonadotropin (hCG) suggest that in conjunction with monoclonal antibodies against hCG, the reported SPR imaging sensor is capable of detecting hCG at concentrations lower than 500 ng/ml. (c) 2004 Elsevier B.V. All rights reserved.