Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors
Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors
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DOI：
10.1016/s1074-7613(00)80082-5
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发表时间：
1999-07-01
期刊：
影响因子：
32.4
通讯作者：
Raulet, DH
中科院分区：
文献类型：
--
作者：
Hanke, T;Takizawa, H;Raulet, DH
小鼠NK细胞表达至少7种抑制性Ly 49受体。在这里，我们采用了半定量细胞-细胞粘附试验以及I类/肽四聚体提供了一个全面的分析Ly 49受体的特异性I类MHC分子在8个MHC单倍型。不同的Ly 49受体表现出不同的结合特性。I类结合的程度与功能抑制的程度有关。四聚体的研究表明，无论是糖基化，也没有coreceptors是必要的I类结合Ly 49受体和发现肽特异性识别Ly 49受体。这些结果为解释和整合许多现有的功能研究以及设计NK细胞发育和自身耐受性的测试提供了基础。
Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell-cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.