Computer-based analysis of the optokinetic response in zebrafish larvae.
Computer-based analysis of the optokinetic response in zebrafish larvae.
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DOI：
10.1101/pdb.prot4961
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发表时间：
2008-03-01
期刊：
影响因子：
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通讯作者：
Neuhauss, Stephan C F
中科院分区：
文献类型：
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作者：
Hodel, Corinne;Neuhauss, Stephan C F
简介 视野中的大幅运动会引发脊椎动物的刻板眼球运动。这种光动反应 (OKR) 在受精后 4 天以上 (dpf) 的斑马鱼 (Danio rerio) 幼虫中非常强大，因此非常适合用于评估遗传操作后的视觉表现。该协议描述了一种简单的方法，通过该方法将计算机生成的视觉刺激投射到屏幕上并由固定的幼虫观看。使用数码摄像机和适当的计算机软件自动记录和评估由此产生的眼球运动。根据所使用软件的复杂程度，可以在录制会话期间实时进行评估，或者通过离线分析录制的电影文件来进行评估。
INTRODUCTIONLarge movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response (OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and appropriate computer software. Depending on the sophistication of the software used, the evaluation can be performed in real time during the recording session, or by analyzing the recorded movie files off-line.