BINDING AND TRANSPORT OF GANGLIOSIDES BY PROSAPOSIN
BINDING AND TRANSPORT OF GANGLIOSIDES BY PROSAPOSIN
复制标题
DOI：
10.1073/pnas.89.23.11254
复制
发表时间：
1992-12-01
影响因子：
11.1
通讯作者：
OBRIEN, JS
中科院分区：
文献类型：
--
作者：
HIRAIWA, M;SOEDA, S;OBRIEN, JS
鞘脂激活蛋白A、B、C和D的前体鞘脂激活蛋白原，其激活鞘脂的溶酶体水解，存在于各种组织和体液中，并且在神经系统中尤其丰富。鞘脂激活蛋白原和鞘脂激活蛋白A、B、C和D与13种不同的神经节苷脂形成稳定的复合物，如通过使用柱色谱的测定所测量的。神经节四糖型神经节苷脂（a系列）以高亲和力结合，而B系列神经节苷脂、O-乙酰化神经节苷脂和具有较短糖链的神经节苷脂以较低亲和力结合。鞘脂激活蛋白原和鞘脂激活蛋白将神经节苷脂从供体脂质体转移到红细胞血影膜。鞘脂激活蛋白原也比成熟鞘脂激活蛋白更能刺激神经节苷脂GM 1 β-半乳糖苷酶。鞘脂激活蛋白原作为一种分泌蛋白和一个完整的膜蛋白存在，我们建议，鞘脂激活蛋白原是活跃的神经节苷脂结合和转运蛋白在体内。
Prosaposin, the precursor of saposins A, B, C, and D, which activate lysosomal hydrolysis of sphingolipids, exists in various tissues and body fluids and is especially abundant in the nervous system. Prosaposin and saposins A, B, C, and D formed stable complexes with 13 different gangliosides as measured by an assay using column chromatography. Gangliosides of the gangliotetraose type (a series) were bound with high affinity, whereas b series gangliosides, O-acetylated gangliosides, and gangliosides with shorter carbohydrate chains, were bound with lower affinity. Prosaposin and saposins transferred gangliosides from donor liposomes to erythrocyte ghost membranes. Prosaposin also stimulated ganglioside GM1 beta-galactosidase more than mature saposins. Prosaposin exists as a secretory protein and as an integral membrane protein, and we propose that prosaposin is active as a ganglioside binding and transport protein in vivo.